Delivery of Methotrexate and Characterization of Skin Treated by Fabricated PLGA Microneedles and Fractional Ablative Laser

Pharmaceutical Research
Hiep X Nguyen, Ajay K Banga

Abstract

This study investigated in vitro transdermal delivery of methotrexate through dermatomed porcine ear and cadaver human skin treated with poly (D,L-lactide-co-glycolide) acid microneedles or fractional ablative laser. PLGA microneedles were fabricated and characterized using scanning electron microscopy and mechanical assessment techniques. The integrity of treated skin was evaluated by rheometer, transepidermal water loss, and skin electrical resistance measurements. Successful skin microporation was demonstrated by dye binding, histology, pore uniformity, confocal laser microscopy, and DermaScan studies. In vitro permeation experiment was performed on Franz diffusion cells to determine drug delivery into and across the skin. Both physical treatments resulted in a considerable decrease in skin resistance and an increase in transepidermal water loss value. The laser-created microchannels were significantly larger than those formed by microneedles (p < 0.05). An effective force of 41.04 ± 18.33 N was required to achieve 100% penetration efficiency of the microneedles. For both porcine ear and human skin, laser ablation provided a significantly higher methotrexate permeability into the receptor chamber and skin layers compared to ...Continue Reading

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Citations

Aug 8, 2018·Pharmaceutics·Hiep X Nguyen, Ajay K Banga
Oct 24, 2018·Drug Development and Industrial Pharmacy·Saili DharadharVandana Patravale
Aug 8, 2019·Expert Opinion on Drug Delivery·Chien-Yu HsiaoJia-You Fang
Jun 25, 2020·Pharmaceutics·Antonio José GuillotAna Melero
Oct 31, 2019·Dose-response : a Publication of International Hormesis Society·Xiaoxiang HeDaming Wu
Jun 18, 2020·International Journal of Nanomedicine·Yun-Chun ZhaoCai-Hong Zheng
Nov 17, 2020·International Journal of Pharmaceutics·Sonalika A BhattaccharjeeAjay K Banga
Sep 9, 2020·International Journal of Pharmaceutics·Madhura KaleAjay K Banga
Jul 18, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ismaiel A TekkoRyan F Donnelly
Apr 26, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ling LiangXin Dong Guo
Dec 20, 2019·Molecular Therapy. Nucleic Acids·Woan-Ruoh LeeJia-You Fang

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Methods Mentioned

BETA
confocal microscopy
confocal laser microscopy

Software Mentioned

Texture Analyzer
Excel Worksheet
[UNK]
DermaScan®
ImageJ
DermaScan
Fluoropore
DermaScan C V3
SPSS
LabSolution IR

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