Delivery of molecules into cells using carbon nanoparticles activated by femtosecond laser pulses.

Nature Nanotechnology
Prerona ChakravartyMark R Prausnitz

Abstract

A major barrier to drug and gene delivery is crossing the cell's plasma membrane. Physical forces applied to cells via electroporation, ultrasound and laser irradiation generate nanoscale holes in the plasma membrane for direct delivery of drugs into the cytoplasm. Inspired by previous work showing that laser excitation of carbon nanoparticles can drive the carbon-steam reaction to generate highly controlled shock waves, we show that carbon black nanoparticles activated by femtosecond laser pulses can facilitate the delivery of small molecules, proteins and DNA into two types of cells. Our initial results suggest that interaction between the laser energy and carbon black nanoparticles may generate photoacoustic forces by chemical reaction to create transient holes in the membrane for intracellular delivery.

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Citations

Nov 25, 2011·ACS Nano·Jun GeRichard N Zare
Jun 1, 2013·Nature Protocols·Maciej AntkowiakFrank J Gunn-Moore
Aug 13, 2013·Advanced Drug Delivery Reviews·Ravi Singh, Suzy V Torti
Jan 17, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Prerona ChakravartyMark R Prausnitz
Jan 18, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Stavros N MoysidisJeffrey L Goldberg
Jan 25, 2014·Scientific Reports·Mischa SchmidtMartin O Steinhauser
Sep 4, 2014·Biotechnology and Bioengineering·Aritra SenguptaMark R Prausnitz
Jun 18, 2015·Nanomedicine·Aritra SenguptaMark R Prausnitz
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Feb 24, 2016·Scientific Reports·Shigehiko Yumura
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Methods Mentioned

BETA
confocal microscopy
flow cytometry
transfection
Assay

Software Mentioned

MINITAB

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