Abstract
Ternary nanocomplexes, composed of bifunctional cationic peptides, lipids and siRNA, as delivery vehicles for siRNA have been investigated. The study is the first to determine the optimal sequence and architecture of the bifunctional cationic peptide used for siRNA packaging and delivery using lipopolyplexes. Specifically three series of cationic peptides of differing sequence, degrees of branching and cell-targeting sequences were co-formulated with siRNA and vesicles prepared from a 1 : 1 molar ratio of the cationic lipid DOTMA and the helper lipid, DOPE. The level of siRNA knockdown achieved in the human alveolar cell line, A549-luc cells, in both reduced serum and in serum supplemented media was evaluated, and the results correlated to the nanocomplex structure (established using a range of physico-chemical tools, namely small angle neutron scattering, transmission electron microscopy, dynamic light scattering and zeta potential measurement); the conformational properties of each component (circular dichroism); the degree of protection of the siRNA in the lipopolyplex (using gel shift assays) and to the cellular uptake, localisation and toxicity of the nanocomplexes (confocal microscopy). Although the size, charge, structur...Continue Reading
References
Jan 1, 1995·Methods in Enzymology·R W Woody
Jan 1, 1995·Biopolymers·G Siligardi, A F Drake
Feb 7, 1997·Science·J O RädlerC R Safinya
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·B PitardJ Crouzet
Nov 23, 2000·Bioconjugate Chemistry·D L McKenzieK G Rice
Oct 28, 2004·Journal of Drug Targeting·Michele J WriterStephen L Hart
Mar 18, 2005·The Journal of Gene Medicine·Qixin LengA James Mixson
May 26, 2005·Nucleic Acids Research·Martin L ReadLeonard W Seymour
Jan 12, 2007·Molecular BioSystems·John J TurnerMichael J Gait
Feb 27, 2007·Journal of Biochemical and Biophysical Methods·Ya-Hui WangHan-Jung Lee
Jun 19, 2007·Nature·Priti KumarN Manjunath
Jun 26, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dana J GaryYou-Yeon Won
Oct 16, 2007·Biochemistry·M Firouz Mohd MustapaAlethea B Tabor
May 17, 2008·Cancer Gene Therapy·Q LengA J Mixson
May 22, 2008·Molecular BioSystems·John B WongHelen C Hailes
Jul 5, 2008·Organic & Biomolecular Chemistry·Christopher A HurleyHelen C Hailes
Aug 12, 2008·Cell·Priti KumarPremlata Shankar
Dec 5, 2008·Chemical Reviews·Meredith A Mintzer, Eric E Simanek
Jan 31, 2009·Nature Reviews. Drug Discovery·Kathryn A WhiteheadDaniel G Anderson
Feb 21, 2009·Bioconjugate Chemistry·M Firouz Mohd MustapaAlethea B Tabor
Apr 23, 2009·Advanced Drug Delivery Reviews·Tamaki Endoh, Takashi Ohtsuki
May 23, 2009·British Journal of Pharmacology·Patrick MidouxPaul-Alain Jaffrès
Jan 19, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sung Wook KimSungho Shin
Mar 6, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephanie M GrosseStephen L Hart
Mar 11, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sang-Soo KimN Manjunath
Apr 16, 2010·Pharmaceutical Research·Bérangère Langlet-BertinAntoine Kichler
May 4, 2010·International Journal of Pharmaceutics·Keerti JainN K Jain
Nov 9, 2010·Molecular BioSystems·Laila KudsiovaM Jayne Lawrence
Jun 1, 2011·Biomaterials·Aristides D TagalakisStephen L Hart
Aug 6, 2011·Molecular Pharmaceutics·Laila KudsiovaM Jayne Lawrence
Aug 6, 2011·Cancer Gene Therapy·S-T ChouA J Mixson
Jan 3, 2012·Biomaterials·Hamidreza M AliabadiHasan Uludağ
Oct 31, 2012·PloS One·Orestis ArgyrosRichard Paul Harbottle
Dec 6, 2012·Molecular Pharmaceutics·Katharina WelserAlethea B Tabor
Jan 31, 2013·Biomacromolecules·Szu-Ting ChouA James Mixson
Citations
Oct 7, 2016·Chemical Communications : Chem Comm·Kejin ZhouDaniel J Siegwart
Feb 2, 2019·Molecular Omics·Ali AlazzoCameron Alexander
May 19, 2018·Science·Matthew C FisherSarah J Gurr
Aug 14, 2020·Bioengineering·Milad AshrafizadehPooyan Makvandi
May 6, 2019·Nanomedicine·Anjuman A BegumPeter M Moyle
Jul 17, 2019·Current Medicinal Chemistry·Fei PengFeng Qiu
Jan 5, 2021·Applied Microbiology and Biotechnology·Kai MaTian-Yun Wang
Nov 4, 2021·Nanoscale·Robin BofingerAlethea B Tabor