Delivery of Small Molecule EF2 Kinase Inhibitor for Breast and Pancreatic Cancer Cells Using Hyaluronic Acid Based Nanogels

Pharmaceutical Research
Ferah Comert OnderBulent Ozpolat

Abstract

To evalauted natural polymeric biomaterials including hyaluronic acid (HA) and its copolymeric form HA:Suc nanoparticles (NPs) as drug carrier systems for delivery of hydrophobic small molecule kinase EF2-kinase inhibitor in breast and pancreatic cancer cells. In vitro cellular uptake studies of Rhodamine 6G labaled HA:Suc nanoparticles were evaluated by using flow cytometry analysis and fluorescent microscopy in breast (MDA-MB-231 and MDA-MB-436) and pancreatic cancer cells (PANC-1 and MiaPaca-2). Besides, in vitro release study of compound A (an EF2-kinase inhibitor) as a model hydrophobic drug was performed in the cancer cells. These biological evaluation studies indicated that HA and HA:Suc NPs provided a highly effective delivery of compound A were into breast and pancreatic cancer cells, leading to significant inhibition of cell proliferation and colony formation of breast and pancreatic cancer cells. HA-sucrose NPs incorporating an EF2-Kinase inhibitor demonstrate significant biologic activity in breast and pancreatic cancer cells. This is the first study that shows natural polymeric drug carriers succesfully deliver a hydrofobic cancer drug into cancer cells. Graphical Abstract Nanoparticles based on HA:Suc are effectiv...Continue Reading

References

Apr 21, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shuang CaiM Laird Forrest
Dec 20, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gaurav BajajYoon Yeo
Nov 7, 2013·Apoptosis : an International Journal on Programmed Cell Death·Ahmed A AshourBulent Ozpolat
Apr 1, 2017·Breast Cancer Research and Treatment·Zuhal HamurcuBulent Ozpolat
May 12, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Recep BayraktarBulent Ozpolat
Aug 17, 2018·Colloids and Surfaces. B, Biointerfaces·Yu Ri ChoiSung-Wook Choi
Oct 1, 2018·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Haci Ahmet BircanBulent Ozpolat
Nov 1, 2018·Physiological Research·J Kousalová, T Etrych
Jan 10, 2019·International Journal of Biological Macromolecules·Selin Sagbas SunerNurettin Sahiner
Feb 10, 2019·Pharmaceutics·Parisa EslamiStefano Fedeli

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Citations

Apr 4, 2021·Biomolecules·Aikaterini BerdiakiDragana Nikitovic

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
fluorescence imaging
Fluorescence

Software Mentioned

ImageJ

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