PMID: 2101483Sep 1, 1990Paper

Delivery of the cholera toxin B subunit by using a recombinant Yersinia enterocolitica strain as a live oral carrier

Research in Microbiology
M P Sory, G R Cornelis

Abstract

The gene ctxB encoding the cholera toxin B subunit was subcloned to design its production by Yersinia enterocolitica. It was joined in two ways to yopH, a gene of the virulence plasmid pYV specific to this genus. This gene encodes one of the major Yop proteins (YopH) secreted by bacteria incubated at 37 degrees C in a Ca(2+)-deprived medium. In a first construction, an operon fusion was obtained between ctxB and yopH so that CT-B and a truncated YopH protein were produced. The recombinant CT-B from Y. enterocolitica was structurally and antigenically similar to CT-B produced by Vibrio cholerae. In another construction, the fusion gene obtained directed the production of YopH'/CT-B hybrid proteins that were secreted by Y. enterocolitica. In both cases, Y. enterocolitica directed the production of the recombinant proteins only when the bacteria were incubated in conditions of Yops production. When bacteria carrying the operon fusion were given orally to mice, a clear serum antibody response against CT-B was detected by ELISA. According to immunoblot analysis, this response was only directed against the polymeric form of the B subunit.

References

Jul 6, 1989·The New England Journal of Medicine·T L Cover, R C Aber
May 1, 1989·The British Journal of Surgery·S E AttwoodF B Keane
Jun 1, 1989·Microbial Pathogenesis·J E Galán, R Curtiss
Oct 1, 1989·Molecular Microbiology·G R CornelisJ C Vanooteghem
Jun 1, 1988·Microbial Pathogenesis·M P Sory, G Cornelis
Jan 1, 1987·Reviews of Infectious Diseases·G CornelisG Wauters
Aug 25, 1982·Nucleic Acids Research·M L GennaroD A Broadbent
Feb 24, 1984·Journal of Immunological Methods·C O ElsonJ Lefkowitz
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G D Pearson, J J Mekalanos

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Citations

Dec 1, 1996·Molecular Biotechnology·D C MarchionR C Judd
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·P WattiauG R Cornelis
Apr 9, 2001·Clinical Microbiology Reviews·P L OgraR C Welliver
Dec 21, 2010·PloS One·Salome Casutt-MeyerGuy R Cornelis
Aug 25, 2009·Molecular Immunology·Maria V CarrollRobert B Sim
Jun 28, 2011·Advanced Drug Delivery Reviews·Maria V Carroll, Robert B Sim
Dec 31, 1996·FEMS Immunology and Medical Microbiology·S G FenwickM Gwozdz

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