Delta-like 3 localizes to neuroendocrine cells and plays a pivotal role in gastrointestinal neuroendocrine malignancy

Cancer Science
Kentaro MatsuoKazuhisa Uchiyama

Abstract

Delta-like 3 (DLL3) is a member of the Delta/Serrate/Lag2 (DSL) group of Notch receptor ligands. Five DSL ligands are known in mammals, among which DLL3 has a unique structure. In the last few years, DLL3 has attracted attention as a novel molecular targeting gene in neuroendocrine carcinoma of the lung due to its high expression. However, the expression pattern and functions of DLL3 in the gastrointestinal tract and gastrointestinal neuroendocrine carcinoma remain unclear. In this study, we examined the expression and role of DLL3 in the gastrointestinal tract, as well as in gastrointestinal neuroendocrine carcinoma. Immunohistochemical staining of the human normal gastrointestinal tract revealed that DLL3 localized in neuroendocrine cells. DLL3 showed intense staining in chromogranin A-positive gastric cancer specimens. Real-time quantitative RT-PCR and western blotting analyses showed considerable upregulation of DLL3 in gastrointestinal neuroendocrine carcinoma cell lines. Immuno-electron microscopy demonstrated abundant expression of DLL3 in neurosecretory granules in these cells. Furthermore, gene silencing of DLL3 caused significant growth inhibition through the induction of intrinsic apoptosis. Our findings suggest that...Continue Reading

References

Jul 30, 1993·International Journal of Cancer. Journal International Du Cancer·T FujiwaraT Nagatsu
Oct 23, 2003·Nature Reviews. Cancer·Freddy Radtke, Kenneth Raj
Aug 1, 2007·The Journal of Cell Biology·Insa GeffersAchim Gossler
Sep 13, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kathleen M LoomesKenro Kusumi
Sep 2, 2008·Oncogene·B D'SouzaG Weinmaster
Aug 12, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Catharina M KorseJohannes M G Bonfrer
Jan 23, 2013·International Journal of Oncology·Kentaro MaemuraYoshinori Otsuki
Mar 1, 2016·Archives of Medical Science : AMS·Paweł GutMarek Ruchała
Dec 23, 2016·Biochemical and Biophysical Research Communications·San-Ming DengHua Han
Mar 21, 2018·Scientific Reports·Hiroki HamamotoKazuhisa Uchiyama
Sep 21, 2018·Drugs in R&D·Bilal H LashariDipesh Uprety
Nov 7, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marissa SpinoAndrew S Chi
Mar 16, 2019·Cancer Science·Megumi FurutaJun Sakakibara-Konishi

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Citations

Jun 18, 2020·International Journal of Molecular Sciences·Rossella GrattonSergio Crovella
May 23, 2020·OncoTargets and Therapy·Meng-Xi XiuBo-Hai Kuang
Jun 20, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Susanne HippPaul J Adam
Feb 20, 2021·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Fiona BlackhallNiels Reinmuth
Jun 30, 2021·Technology in Cancer Research & Treatment·Qi LiuZhiyong Yu

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Methods Mentioned

BETA
histone acetylation
transmission electron microscopy
Protein Assay
transfection
fluorescence microscopy
Assay
electron microscopy
electron

Software Mentioned

LAS X

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