PMID: 11923099Mar 30, 2002Paper

Delta6-, Stearoyl CoA-, and Delta5-desaturase enzymes are expressed in beta-cells and are altered by increases in exogenous PUFA concentrations

Biochimica Et Biophysica Acta
Sasanka RamanadhamJohn Turk

Abstract

In the evolution of Type II diabetes, an initial period of hyper-fatty acidemia leads to an insulin secretory defect which triggers overt hyperglycemia and frank diabetes. The mechanism by which elevated free fatty acids contribute to beta-cell dysfunction, however, is not clearly understood. We recently reported that arachidonic acid (20:4) or linoleic acid (18:2) supplementations result in increases in abundances of long chain polyunsaturated fatty acids in INS-1 beta-cell membrane lipids, suggesting that beta-cells express desaturases that catalyze generation of unsaturated fatty acids. As expression of desaturases by beta-cells has not yet been addressed, we initiated studies to examine this issue using INS-1 beta-cells and find that they express messages for the Delta6-, stearoyl CoA-, and Delta5-desaturase. Supplementation of the INS-1 beta-cells with arachidonic acid leads to decreased expression of all three desaturases, presumably in response to the decreased need for endogenous generation of unsaturated fatty acids. In contrast, linoleic acid supplementation promoted minimal changes in the three desaturases. These findings demonstrate for the first time that beta-cells express regulatable desaturases. Additionally, re...Continue Reading

References

May 1, 1979·Diabetes·M G EckF H Faas
Apr 30, 1992·Biochemical and Biophysical Research Communications·S RamanadhamJ Turk
Mar 25, 1992·Biochimica Et Biophysica Acta·M L BlankF Snyder
May 1, 1991·Molecular and Cellular Endocrinology·R A IgalI N de Gómez Dumm
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B L Scott, N G Bazan
Sep 1, 1988·Scandinavian Journal of Clinical and Laboratory Investigation·T A Hagve
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R T HolmanD M Brown
Dec 1, 1984·Proceedings of the Society for Experimental Biology and Medicine·P J Wilder, J G Coniglio
Nov 16, 1981·Biochemical and Biophysical Research Communications·C GrunfeldC R Kahn
Jan 1, 1995·Progress in Lipid Research·J M Ntambi
Jul 1, 1993·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·S LadhaK Bowler
Jan 28, 1993·The New England Journal of Medicine·M BorkmanL V Campbell
Mar 1, 1993·Diabetes·J TurkS Ramanadham
Jun 1, 1996·Diabetologia·L H StorlienL V Campbell
Mar 28, 1997·The Journal of Biological Chemistry·J TangS S Jones
Jul 15, 1997·The Journal of Clinical Investigation·M ShimabukuroR H Unger
Oct 6, 1997·The Journal of Clinical Investigation·M ShimabukuroR H Unger
Mar 7, 1998·The Journal of Biological Chemistry·M ShimabukuroR H Unger
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M ShimabukuroR H Unger

❮ Previous
Next ❯

Citations

Dec 9, 2004·American Journal of Physiology. Endocrinology and Metabolism·Michael J MacDonaldMindy A Kendrick

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.