DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases.

Proceedings of the National Academy of Sciences of the United States of America
Teresa Morales-RuizTeresa Roldán-Arjona

Abstract

Cytosine methylation is an epigenetic mark that promotes gene silencing and plays important roles in development and genome defense against transposons. Methylation patterns are established and maintained by DNA methyltransferases that catalyze transfer of a methyl group from S-adenosyl-L-methionine to cytosine bases in DNA. Erasure of cytosine methylation occurs during development, but the enzymatic basis of active demethylation remains controversial. In Arabidopsis thaliana, DEMETER (DME) activates the maternal expression of two imprinted genes silenced by methylation, and REPRESSOR OF SILENCING 1 (ROS1) is required for release of transcriptional silencing of a hypermethylated transgene. DME and ROS1 encode two closely related DNA glycosylase domain proteins, but it is unknown whether they participate directly in a DNA demethylation process or counteract silencing through an indirect effect on chromatin structure. Here we show that DME and ROS1 catalyze the release of 5-methylcytosine (5-meC) from DNA by a glycosylase/lyase mechanism. Both enzymes also remove thymine, but not uracil, mismatched to guanine. DME and ROS1 show a preference for 5-meC over thymine in the symmetric dinucleotide CpG context, where most plant DNA met...Continue Reading

References

Jan 1, 1975·Cytogenetics and Cell Genetics·A D Riggs
May 16, 1991·Nature·D FrankH Cedar
May 31, 1996·The Journal of Biological Chemistry·P NeddermannJ Jiricny
Aug 1, 1997·Trends in Genetics : TIG·J A YoderT H Bestor
Jun 22, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·V Colot, J L Rossignol
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·B ZhuJ P Jost
Jun 29, 2000·Annual Review of Biochemistry·A K McCulloughR S Lloyd
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·B ZhuJ P Jost
Aug 11, 2001·Science·W ReikJ Walter
Jan 10, 2002·Genes & Development·Adrian Bird
Jan 12, 2002·Plant Molecular Biology·M V García-OrtizT Roldán-Arjona
Oct 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sugiko WatanabeMitsuyoshi Nakao
Nov 25, 2003·Science·Tetsu KinoshitaTetsuji Kakutani
Feb 6, 2004·The Journal of Biological Chemistry·Varsha S LikhiteAnn M Nardulli
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·E. J. FinneganE. S. Dennis
May 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yeonhee ChoiRobert L Fischer
Dec 1, 2004·Annual Review of Genetics·Deborah E Barnes, Tomas Lindahl
Feb 24, 2005·Annual Review of Plant Biology·Judith Bender
Mar 16, 2005·Biochemistry·Na Li, Faqing Huang
Apr 12, 2005·Science·Uwe H F Bunz
Apr 13, 2005·Biochemistry·Mikael Lund, Bo Jönsson
Oct 12, 2005·Biochemistry·Ishac Nazi, Gerard D Wright

❮ Previous
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Citations

May 31, 2013·Plant Cell Reports·Pranav Pankaj SahuManoj Prasad
Oct 8, 2008·Chromosoma·Yoko Ikeda, Tetsu Kinoshita
Oct 6, 2009·Functional & Integrative Genomics·Sadegh Azimzadeh Jamalkandi, Ali Masoudi-Nejad
May 22, 2008·Plant Molecular Biology·Ana Pilar Ortega-GalisteoTeresa Roldán-Arjona
Jan 15, 2013·Plant Molecular Biology·Klaus Humbeck
Sep 3, 2013·DNA Repair·Ángel Ramiro-MerinaTeresa Roldán-Arjona
Apr 27, 2013·Trends in Plant Science·Raja RagupathySylvie Cloutier
Feb 16, 2011·Cell Research·Xin-Jian HeJian-Kang Zhu
Dec 10, 2009·Heredity·C Köhler, I Weinhofer-Molisch
May 13, 2010·Heredity·F K Teixeira, V Colot
Feb 10, 2010·Nature Reviews. Genetics·Julie A Law, Steven E Jacobsen
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Susan C Wu, Yi Zhang
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Young Geun MokJin Hoe Huh
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Honggui LaGuo-Liang Wang
Apr 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jessica A RodriguesDaniel Zilberman
May 22, 2010·The Journal of Biological Chemistry·María Isabel Ponferrada-MarínRafael R Ariza
Nov 3, 2010·Nucleic Acids Research·María Isabel Ponferrada-MarínRafael R Ariza
Sep 25, 2012·Nucleic Acids Research·Marloes L de GrooteMarianne G Rots
Oct 5, 2012·Nucleic Acids Research·María Isabel Ponferrada-MarínRafael R Ariza
Jul 23, 2013·Nucleic Acids Research·Jara Teresa Parrilla-DoblasRafael R Ariza
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·M MatzkeA J M Matzke
Dec 1, 2012·Cold Spring Harbor Symposia on Quantitative Biology·H Zhang, J-K Zhu
Oct 24, 2007·Plant Physiology·Jon PentermanRobert L Fischer
Jul 22, 2010·Development, Growth & Differentiation·Ming ChenYijun Meng
Aug 8, 2009·Annual Review of Genetics·Jian-Kang Zhu
Nov 2, 2013·BMC Plant Biology·Aliki KapazoglouAthanasios S Tsaftaris
Jun 14, 2013·Plant Methods·Thomas J Hardcastle
Sep 4, 2009·Genome Biology·Mark A Johnson, Judith Bender
Aug 15, 2008·PLoS Biology·Pauline E JullienFrédéric Berger
Aug 30, 2008·PLoS Biology·Liliana M Costa, José F Gutierrez-Marcos
Jul 24, 2010·Yi chuan = Hereditas·Hong-Yu ZhangXian-Jun Wu
Aug 28, 2012·Epigenomics·Shannon R Dalton, Alfonso Bellacosa

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