Demethoxycurcumin-carrying chitosan-antibody core-shell nanoparticles with multitherapeutic efficacy toward malignant A549 lung tumor: from in vitro characterization to in vivo evaluation

Molecular Pharmaceutics
Wei-Ting HuangDean-Mo Liu

Abstract

Targeting controlled release core-shell nanocarriers with the potential to overcome multidrug resistant (MDR) lung cancer were prepared based on demethoxycurcumin (DMC) loaded amphiphilic chitosan nanoparticles coated with an anti-EGFR antibody layer. The nanocarriers were characterized with regard to size with dynamic light scattering, SEM, and TEM. The characterization confirmed the nanocarriers to have a surface coating of the anti-EGFR antibody and a final size excellently suited for circulating targeting nanocarriers, i.e., <200 nm in diameter. In vitro drug release revealed extended quasi-Fickian release from the nanocarriers, with the anti-EGFR layer further reducing the release rate. Cell culture experiments using normoxic and MDR hypoxic cells overexpressing EGFR confirmed improved DMC delivery for anti-EGFR coated particles and revealed that the DMC was delivered to the cytoplasmic region of the cells, forming nanoprecipitates in lysosomes and endosomes. The effective endocytosis and targeting of the core-shell nanoparticles resulted in the nanocarriers achieving high cytotoxicity also against MDR cells. The therapeutic potential was further confirmed in an A549 xenograft lung tumor mouse model, where DMC loaded core-...Continue Reading

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Citations

Oct 30, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Wei-Ting HuangGuang-Yuh Chiou
Oct 21, 2016·Expert Opinion on Drug Delivery·Wing-Hin LeeRamin Rohanizadeh
Oct 13, 2017·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Ali DabbaghNoorsaadah Abdul Rahman
Apr 21, 2020·Phytotherapy Research : PTR·Mahsa SalehiHamed Mirzaei
Oct 8, 2016·Nanoscale·Kegang LiuPatrick Hunziker
Mar 20, 2021·Macromolecular Bioscience·Asim MushtaqLisbeth Grøndahl
May 22, 2021·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Tony JinAudrey Moores
Sep 28, 2021·International Journal of Pharmaceutics·Phuoc Vinh NguyenEmilie Allard-Vannier

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