Demethylation of a nonpromoter cytosine-phosphate-guanine island in the aromatase gene may cause the aberrant up-regulation in endometriotic tissues

Fertility and Sterility
Masao IzawaTasuku Harada

Abstract

To search for the demethylated cytosine-phosphate-guanine (CpG) islands within the aromatase gene in stromal cells derived from endometriotic chocolate cysts. Prospective study. Department of Obstetrics and Gynecology and Department of Biosignaling, Tottori University, Yonago, Japan. Twenty-eight women who underwent laparoscopy (n=14) and laparotomy (n=14). Endometrial and endometriotic stromal cells were obtained from the uterus and chocolate cyst lining of the ovary. We searched for the CpG island and examined methylation profile and the association of methyl-binding proteins with the CpG island. Up-regulation of aromatase messenger RNA (mRNA) expression was demonstrated in endometriotic cells. Three proximal promoters drove the mRNA expression. In endometrial cells, a marginal level of aromatase mRNA expression was observed. Treating endometrial cells with the demethylating agent 5-aza-2'-deoxycytidine markedly enhanced aromatase mRNA expression. The same promoters as in the endometriotic cells were used. To identify the unmethylated CpGs in endometriotic cells, we searched for CpG islands within the aromatase gene and subsequently examined the methylation profiles. Sequence analysis of bisulfite-treated genomic DNA demonstr...Continue Reading

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Citations

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