Demethylation of repetitive DNA sequences in neuroblastoma

Genes, Chromosomes & Cancer
D ThoravalS M Hanash

Abstract

Altered genomic methylcytosine content has been described for a number of tumor types, including neuroblastoma. However, it remains to be determined for different tumor types whether specific loci or chromosomal regions are affected by a methylation change or whether the change is random. We have implemented a computer-based approach for the analysis of two-dimensional separations of human genomic restriction fragments. Through the use of methylation-sensitive restriction enzymes, methylation differences in genomic DNA between tumor and normal tissues can be detected. We report the cloning and sequencing of two fragments detectable in two-dimensional separations of genomic DNA of neuroblastomas. These fragments were found to be a part of repetitive units that exhibited demethylation in neuroblastoma relative to other tumor types. Our finding of a distinct pattern of methylation of repetitive units in neuroblastoma suggests that altered methylation at certain loci may contribute to the biology of this tumor.

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Citations

Oct 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Melanie Ehrlich
Dec 1, 2005·Molecular Cancer Research : MCR·Rie NishiyamaMelanie Ehrlich
May 29, 2009·BMC Medical Genomics·Alexey N KatarginNatalia P Kisseljova
Apr 1, 2010·Epigenomics·Jason P RossPeter L Molloy
Aug 3, 2002·Oncogene·Dominic J Smiraglia, Christoph Plass
Aug 3, 2002·Oncogene·Melanie Ehrlich
Apr 21, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Gabrijela DumbovicManuel Perucho
May 4, 2020·Methods : a Companion to Methods in Enzymology·Gabrijela DumbovićSonia-V Forcales
Jun 5, 2021·Epigenetics & Chromatin·Xena Giada Pappalardo, Viviana Barra
Jun 5, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Shinichi NakagawaTetsuro Hirose

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