PMID: 9180369Jan 1, 1997Paper

Demonstration and purification of a myristoyl-CoA binding protein from bovine cardiac muscle

Life Sciences
R V Raju, R Sharma

Abstract

Protein myristoylation refers to the co-translational addition of myristoyl group to an amino-terminal glycine residue of a protein by the enzyme myristoyl-CoA:protein N-myristoyltransferase (NMT). The myristoylation reaction depends on the availability of the cellular pools of coenzyme A and myristate and their subsequent formation of myristoyl-CoA, the substrate of NMT. In the present study a myristoyl-CoA binding protein (MCBP) was purified using various column chromatographies: hydroxylapatite, DEAE Sepharose CL-6B and Sephacryl S-300 gel filtration. The purified protein exhibited an apparent molecular mass of 50 kDa on SDS-polyacrylamide gel electrophoresis. Incubation of protein with [1-(14)C]myristoyl-CoA followed by denaturing gel electrophoresis, fluorography and treatment with hydroxylamine yielded results that are highly suggestive of a covalent ester-linked acyl-protein complex. This complex formation was not observed in the crude cytosolic fractions. The addition of cytosolic fraction to a progressing acyl-protein complex, resulted in deacylation suggesting a role for thioesterase or/proteinases in the regulation of the acylation reaction in bovine cardiac muscle. The acyl-protein complex formation was not inhibite...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R J DuronioJ I Gordon
Dec 1, 1991·Analytical Biochemistry·M J King, R K Sharma
Apr 18, 1986·Science·B Merrifield
Jan 1, 1983·Current Topics in Microbiology and Immunology·M F Schmidt
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S A CarrK Titani
Nov 1, 1995·Journal of the National Cancer Institute·B A MagnusonR K Sharma
Jan 1, 1994·Annual Review of Biochemistry·D R JohnsonJ I Gordon
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J K LodgeJ I Gordon
Jan 5, 1996·Biochimica Et Biophysica Acta·M NocitoP González-Porqué
Aug 1, 1995·Molecular and Cellular Biochemistry·R V RajuR K Sharma
Jun 1, 1996·Protein Expression and Purification·R V RajuR K Sharma

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Citations

Jul 9, 1999·Progress in Lipid Research·J C DeMarR E Anderson
Jul 19, 2006·Progress in Lipid Research·Ponniah SelvakumarRajendra K Sharma
Dec 2, 2004·Canadian Journal of Physiology and Pharmacology·Rajendra K Sharma

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