PMID: 75238Feb 1, 1978Paper

Demonstration and quantitation of activation of the first component of complement in human serum

The Journal of Experimental Medicine
R J Ziccardi, N R Cooper

Abstract

Activation of the first component of human complement (C1) in human sera can be readily detected in double immunodiffusion studies with anti-C1q, anti- C1r, and anti-C1s as it produces a characteristic pattern quite different from that of precursor C1. Native macromolecular C1 gives a continuous line of precipitation with antisera to C1q, C1r, and C1s in double diffusion studies. After activation of C1 by incubation of serum with complement activators, three major changes occurred in the Ouchterlony pattern. First, spurring of the C1s precipitin line over that of macromolecular C1, indicating release of C1s from C1, was observed with low doses of activator. Release of C1s was quantitated by single radial diffusion and shown to be complete with the highest activator dose examined. Second, C1q was released with larger activator doses as shown also by spurring of the precipitin line due to this component over the remaining macromolecular C1. Third, and most surprising, C1r antigenicity was progressively lost as the activator dose was increased and no C1r line remained with the highest dose of activator tested. This was not true with C1s as there was no change in the total C1s concentration in serum incubated with various activator...Continue Reading

References

Jan 1, 1969·Annual Review of Biochemistry·H J Müller-Eberhard
Mar 1, 1974·Arthritis and Rheumatism·M M De Bracco, J A Manni
Nov 1, 1974·The Journal of Experimental Medicine·R D Schreiber, H J Müller-Eberhard
Aug 29, 1972·Biochemistry·M A Calcott, H J Müller-Eberhard
Jan 1, 1968·Annual Review of Medicine·P H Schur, K F Austen
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Oct 1, 1968·The Journal of Experimental Medicine·G B Naff, O S Ratnoff
May 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·I H LEPOWL PILLEMER
Jun 1, 1963·The Journal of Experimental Medicine·I H LEPOWC F HINZ
Apr 1, 1964·The Journal of Experimental Medicine·G B NAFFI H LEPOW
Nov 1, 1964·The Journal of Clinical Investigation·V H DONALDSON, F S ROSEN

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Citations

Jan 1, 1980·Journal of Immunological Methods·E Linder
Jul 1, 1978·The Journal of Experimental Medicine·J ScharfsteinV Nussenzweig
Mar 1, 1981·The Journal of Experimental Medicine·B GhebrehiwetA P Kaplan
May 1, 1983·The Journal of Clinical Investigation·B GhebrehiwetA P Kaplan
Jan 1, 1986·Critical Reviews in Clinical Laboratory Sciences·A P Dalmasso
Dec 1, 1989·Kidney International·J ZwirnerH E Feucht
May 1, 1985·The Journal of Pediatrics·F B WaldoC D West
Jan 25, 1979·Biochimica Et Biophysica Acta·G J ArlaudM G Colomb

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