PMID: 415762Mar 14, 1978Paper

Demonstration of 5'-methylthioadenosine phosphorylase activity in various rat tissues. Some properties of the enzyme from rat lung

Biochimica Et Biophysica Acta
D L Garbers

Abstract

An enzyme (5'-methylthioadenosine phosphorylase) that catalyzes the phosphorolytic cleavage of 5'-methylthioadenosine to 5-methylthioadenosine to 5-methylthioribose-1-phosphate and adenine was found in various rat tissues. Liver and lung had the highest enzyme activities and heart the lowest, most of the activity (greater than 90%) was recovered in soluble tissue fractions. The enzyme from rat lung was purified about 30-fold by pH treatment (NH4)2SO4 fractionation, and gel filtration. The enzyme did not require an added metal-ion for activity, and was not inhibited by EDTA. Many compounds were tested for their inhibitory effects; of these, ribose 1-phosphate, 2-deoxyribose 1-phosphate, fructose 1-phosphate, adenine and guanine were shown to inhibit. Kinetic patterns on reciprocal plots were linear as a function of the concentration of either 5'-methylthioadenosine or phosphate. More detailed kinetic studies suggested that the rat lung 5'-methylioadenosine phosphorylase catalyzes an equilibrium-ordered reaction, and that 5'-methylthioadenosine is the first substrate to bind and 5-methylthioribose-1-phosphate is the first product to be released.

Citations

Mar 14, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·A SahotaT Gibson
Jan 1, 1981·The International Journal of Biochemistry·L ShugartB Chastain
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N KamataniD A Carson
Dec 18, 2001·The Biochemical Journal·Matthew J Pugmire, Steven E Ealick
Feb 1, 1982·Biochemical Pharmacology·H G Williams-AshmanP Galletti
Sep 16, 1981·Biochemical and Biophysical Research Communications·S K Shapiro, A Barrett
Sep 30, 1982·Biochemical and Biophysical Research Communications·P S Backlund, R A Smith
Jun 1, 1983·Archives of Biochemistry and Biophysics·J I Toohey
Nov 1, 1979·European Journal of Biochemistry·M Carteni'-FarinaV Zappia
Nov 19, 1985·Biochemistry·M H IltzschS Cha
Mar 15, 1985·Biochemical and Biophysical Research Communications·P S SunkaraP J Lachman
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·J I Toohey

Related Concepts

Adenocard
Metazoa
Liver
Lung
Pentosyltransferase
Nicotinamide Riboside Phosphorylase
Drug or Chemical Tissue Distribution
Rats, Laboratory

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