PMID: 3115302Sep 25, 1987Paper

Demonstration of a 12-lipoxygenase activity in bovine polymorphonuclear leukocytes

Biochimica Et Biophysica Acta
P WalstraJ F Vliegenthart

Abstract

In this study we present evidence for the existence of an intrinsic 12-lipoxygenase in the bovine polymorphonuclear leukocyte which differs from the well-known platelet 12-lipoxygenase. Intact bovine polymorphonuclear leukocytes synthesize predominantly 5-lipoxygenase products. However, this 5-lipoxygenase activity disappears completely upon sonication of the cells, whereas a 12-lipoxygenase activity then becomes apparent. This 12-lipoxygenase resembles the platelet 12-lipoxygenase in metabolizing arachidonic acid into 12(S)-hydroxyeicosatetraenoic acid and in being independent of Ca2+ as well as of ATP. The most striking difference between the two 12-lipoxygenases is their behaviour towards linoleic acid. While the platelet 12-lipoxygenase does not convert linoleic acid, the 12-lipoxygenase from bovine polymorphonuclear leukocytes, apparent only in the cell-free system, converts linoleic acid into 13-hydroxyoctadecadienoic acid as efficiently as it converts arachidonic acid into 12-hydroxyeicosatetraenoic acid. This provides a convenient method to distinguish both 12-lipoxygenase activities. The fact that this new 12-lipoxygenase is able to metabolize linoleic acid into 13-hydroxyoctadecadienoic acid suggests that this enzyme,...Continue Reading

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Citations

Dec 1, 1992·Prostaglandins·G BioqueE Gelpi
Jan 1, 1991·Free Radical Biology & Medicine·S Yamamoto
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·T Yoshimoto, S Yamamoto
Apr 3, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K Shen, C A Herman
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Mar 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S Yamamoto
Nov 30, 1987·Biochemical and Biophysical Research Communications·P WalstraJ F Vliegenthart
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Apr 1, 1992·Journal of Neurochemistry·M NishiyamaT Kirino
Mar 1, 1997·Progress in Lipid Research·S YamamotoN Ueda
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Jan 1, 1988·Progress in Lipid Research·A A SpectorS A Moore
Sep 22, 1992·Biochimica Et Biophysica Acta·E M van der DonkJ F Vliegenthart
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Nov 1, 1988·Archives of Biochemistry and Biophysics·Y TakahashiS Yamamoto
Jun 15, 1989·Biochemical and Biophysical Research Communications·O ReinaudD Mansuy

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