PMID: 9546677Apr 18, 1998Paper

Demonstration of a Ca2+ requirement for thyroglobulin dimerization and export to the golgi complex

European Journal of Biochemistry
Bruno Di JesoI V Sandoval

Abstract

We have examined the effects of depleting the endoplasmic reticulum Ca2+ store on the maturation of newly synthesized thyroglobulin molecules, their export to the Golgi complex, and their secretion by FRTL-5 cells. An inhibitor of the endoplasmic reticulum Ca2+ pump, thapsigargin, and the Ca2+ ionophore A23187 depleted the endoplasmic reticulum Ca2+ store and strongly inhibited thyroglobulin secretion in cells chased in medium containing 0.1 mM Ca2+. Inhibition of thyroglobulin secretion was caused by a block in the export of newly synthesized thyroglobulin molecules from the endoplasmic reticulum to the Golgi complex, as shown by cell-fractionation experiments and the intracellular accumulation of endoH-sensitive thyroglobulin. The thyroglobulin molecules retained in the endoplasmic reticulum of cells treated with the drugs were found to assemble more slowly into dimers than thyroglobulin in control cells. Protease-sensitivity experiments demonstrated that thyroglobulin dimers assembled in the presence of thapsigargin had a different conformation with respect to dimers assembled in controls cells.

Citations

Sep 2, 1998·Molecular and Cellular Endocrinology·F GentileG Palumbo
Apr 1, 2010·The Journal of Biological Chemistry·Xiaofan WangPeter Arvan
Apr 12, 2014·Pflügers Archiv : European journal of physiology·Kid TörnquistTero Viitanen
Feb 15, 2001·Biochemical Pharmacology·E JiménezM Montiel
Feb 13, 2001·Seminars in Cell & Developmental Biology·M C Ashby, A V Tepikin
Jun 22, 2010·Hearing Research·Qi WangP S Steyger
Jul 18, 2014·Molecular Biology of the Cell·Mark J HendersonBrandon K Harvey
Nov 26, 2015·Endocrine Reviews·Bruno Di Jeso, Peter Arvan
Jan 16, 1999·Scandinavian Journal of Clinical and Laboratory Investigation·T Zimmermann-BelsingU Feldt-Rasmussen
Aug 15, 2009·Chemical Reviews·Peter VangheluweJo Vanoevelen
May 1, 2021·Cancers·Muhammad Yasir AsgharKid Törnquist

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