Demonstration of a peptidoglycan-linked lipoprotein and characterization of its trypsin fragment in the outer membrane of Brucella spp.

Infection and Immunity
M J Gómez-Miguel, I Moriyón

Abstract

The sodium dodecyl sulfate (SDS) extraction-trypsin digestion protocol used by Braun and Sieglin (V. Braun and U. Sieglin, Eur. J. Biochem. 13:336-346, 1970) to show the peptidoglycan-linked lipoprotein of Escherichia coli was applied to both Brucella abortus and E. coli. Whereas a single polypeptide of 8,000 molecular weight was obtained from E. coli, several proteins of apparent molecular weight lower than 35,000 were demonstrated by SDS-polyacrylamide gel electrophoresis in B. abortus. These results did not change when the trypsin digestion conditions were modified. On the other hand, when the SDS extractions were performed under conditions more stringent than those used for other gram-negative bacteria, only a polypeptide fragment of apparent molecular weight of 8,000 was obtained from B. abortus. This polypeptide was similar to the trypsin fragment of the E. coli lipoprotein with respect to its behavior in SDS-polyacrylamide gels, isoelectric point in urea, molecular weight, and presence of both ester- and amide-linked fatty acids. Moreover, the amino acid analysis showed an overall similarity with respect to the amino acid composition of E. coli lipoprotein. A polypeptide of the same molecular weight, isoelectric point, a...Continue Reading

References

Oct 31, 1975·Biochimica Et Biophysica Acta·V Braun
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C R MerrilM L Van Keuren
Jul 1, 1978·Journal of Biochemistry·T Mizuno, M Kageyama
Feb 1, 1978·European Journal of Biochemistry·J GmeinerH H Martin
Nov 15, 1976·European Journal of Biochemistry·V BraunH Hagenmaier
Jun 1, 1976·Infection and Immunity·L M JonesD T Berman
Dec 4, 1972·European Journal of Biochemistry·C GalanosD W Watson
Jan 1, 1969·European Journal of Biochemistry·O W ThieleC Lacave
Jul 13, 1972·European Journal of Biochemistry·E T RietschelO Westphal
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Apr 1, 1967·Journal of Bacteriology·R DiazJ B Wilson
Aug 1, 1983·Analytical Biochemistry·K Hancock, V C Tsang
Apr 1, 1984·Infection and Immunity·J T DouglasA J Winter
Oct 1, 1984·Infection and Immunity·J M SantosA J Winter
Mar 1, 1982·Infection and Immunity·D R VerstreateA J Winter
Jul 1, 1980·Journal of Bacteriology·D BaumgardnerS Kaplan
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Jul 1, 1959·Biochimica Et Biophysica Acta·F SNYDER, N STEPHENS

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Citations

Jan 1, 1990·Critical Reviews in Microbiology·L D Smith, T A Ficht
Oct 26, 2012·The FEBS Journal·Hiroshi NakayamaBok Luel Lee
Aug 24, 1999·Infection and Immunity·A TiborJ J Letesson
Jan 1, 1987·Annales De L'Institut Pasteur. Microbiology·A J Winter
Jan 1, 1987·Annales De L'Institut Pasteur. Microbiology·I MoriyónR Díaz
Mar 1, 1988·Infection and Immunity·M J Gómez-MiguelR Díaz
Sep 1, 1988·Journal of Bacteriology·G A WeinbergR S Munson

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