Demonstration of a specific serum carrier protein of nonsuppressible insulin-like activity in vivo

The Journal of Clinical Endocrinology and Metabolism
U KaufmannE R Froesch

Abstract

The fate of nonsuppressible insulin-like activity (NSILA-S) was studied by injecting a tracer of 125I-NSILA-S iv into rats. Ten minutes after injection of 125I-NSILA-S alone, 20% of the label is found in serum, whereas after the injection of 125-I-insulin or 125I-NSILA-S together with an excess of cold NSILA-S only 8% of the label are recovered. Sephadex G-200 chromatography at neutral pH of serum after injection of 125I-NSILA-S reveals 2 peaks of radioactivity in the high molecular weight region at 67 and 47% bed volume. Five minutes after injection the peak at 67% starts to disappear, whereas the one at 47% persists with a half-life of 3 h. The latter peak was submitted to Sephadex G-200 chromatography at acidic pH which dissociates NSILA-S from its binding protein. The labeled material obtained by this procedure still exhibits the same binding characteristics to chick embryo fibroblasts as standard 125 I-NSILA-S. A third peak at 90% bed volume corresponding to low molecular NSILA-S is no longer detectable 20 min after injection. A fourth peak at 100% bed volume becomes apparent after 1 h. The half-life and chromatographic pattern of iv injected 125 I-NSILA-S are not changed by the simultaneous administration of insulin or gr...Continue Reading

Citations

Jan 1, 1983·The International Journal of Biochemistry·H J Cornell, A C Herington
Jan 1, 1988·Journal of Steroid Biochemistry·M E LippmanA Kasid
Jan 1, 1989·Journal of Steroid Biochemistry·M E Lippman, R B Dickson
Dec 1, 1980·Cell·D Barnes, G Sato
Nov 1, 1979·Molecular and Cellular Endocrinology·R C FranklinA C Herington
Feb 1, 1984·Molecular and Cellular Endocrinology·G L Smith
Jan 1, 1989·Progress in Growth Factor Research·R C Baxter, J L Martin
Oct 1, 1995·Critical Reviews in Oncology/hematology·B NguyenP G Johnston
Feb 14, 1980·The New England Journal of Medicine·L S Phillips, R Vassilopoulou-Sellin
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D J Knauer, G L Smith
Oct 1, 1992·Molecular Biology of the Cell·R Flaumenhaft, D B Rifkin
Dec 1, 1993·Journal of Internal Medicine·E R Froesch, M Hussain
Mar 1, 1988·European Journal of Biochemistry·R RappU Weber
Dec 1, 1986·The Journal of Clinical Investigation·R C Baxter, J L Martin
Jun 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S M Ferkovich, H Oberlander
Jan 1, 1987·Domestic Animal Endocrinology·F C BuonomoD R Campion
Jan 1, 1983·The International Journal of Biochemistry·A C HeringtonA D Kuffer
Sep 1, 1995·Metabolism: Clinical and Experimental·M JeevanandamS R Petersen
Jan 1, 1995·Progress in Growth Factor Research·R L Hintz
May 1, 1990·Trends in Endocrinology and Metabolism : TEM·E R FroeschJ Zapf
Jul 1, 1991·Experimental Eye Research·S F HackettP A Campochiaro
Mar 1, 1984·Clinics in Endocrinology and Metabolism·R L Hintz
Aug 1, 1985·Diabetologia·E R Froesch, J Zapf
Nov 1, 1984·Experimental Cell Research·G Kessler-IceksonL Wasserman
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·D J KnauerG L Smith
Jul 1, 1991·The British Journal of Nutrition·T UmezawaT Noguchi
Sep 1, 1987·Journal of Cellular Biochemistry·M E LippmanA Kasid
Mar 13, 2009·American Journal of Physiology. Cell Physiology·Paulette M Yamada, Kuk-Wha Lee
Jul 1, 1986·Annals of Clinical Biochemistry·J D Teale, V Marks

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