Demonstration of acid hydrolase activity in primary cultures of bovine brain microvessel endothelium

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
A Baranczyk-KuzmaK L Audus

Abstract

The existence of lysosomes and acid hydrolase activity was demonstrated in an in vitro blood-brain barrier (BBB) model comprising primary cultures of bovine brain microvessel endothelial cell (BMEC) monolayers. BMEC lysosomes were observed by the uptake of acridine orange and fluorophore-labeled acetylated low-density lipoprotein by fluorescence microscopy. Cytochemical localization of the acid hydrolase, sulfatase, and acid phosphatase (AcP) activities with light microscopy also revealed hydrolase-positive vacuoles or lysosomes that varied in number from cell to cell. BMEC monolayers were fractionated and biochemical assays of the sulfatase, AcP, and beta-galactosidase were performed. Significant activities of the acid hydrolases were found to be associated with lysosome and microsome fractions (69-77%). The majority of beta-galactosidase (approximately 48%) and total sulfatase (approximately 58%) activity was associated with the lysosome fraction of the BMECs. In contrast, approximately 52% of AcP activity was associated with the microsome fraction of the cells. The results of this study are consistent with the demonstration in vivo of acid hydrolases as potential factors in the endocytic pathway for transport of proteins thr...Continue Reading

References

Dec 1, 1975·The Journal of Cell Biology·E J BrandtR T Swank
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R D BroadwellM Salcman
Oct 1, 1989·Journal of the American Podiatric Medical Association·F L Sapico
Mar 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·I E GoetzD N Krause
Jun 1, 1986·Journal of Neurochemistry·A Baranczyk-KuzmaR T Borchardt
Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M Robinson
Jan 1, 1987·Annals of the New York Academy of Sciences·K L Audus, R T Borchardt
May 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Kawano, E Aikawa
Sep 1, 1987·Cell and Tissue Research·H V VintersS A Moore
Jan 1, 1974·Methods in Enzymology·H Koenig
May 1, 1973·The Biochemical Journal·M WorwoodF A Rose
Jan 1, 1966·Annual Review of Physiology·C De Duve, R Wattiaux
Jul 1, 1970·Archives of Biochemistry and Biophysics·H Chaimovich, F Nome
Jul 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Goldfischer
Oct 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M Robinson, M J Karnovsky
Jan 1, 1984·Neurochemical Research·F Lasbennes, J Gayet
May 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R E PitasR W Mahley
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R D Broadwell, M Salcman
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT

❮ Previous
Next ❯

Citations

Dec 1, 1993·Neurochemistry International·F Joó
Mar 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C A SzabóF Joó
Jan 1, 1992·Journal of Neurochemistry·F Joó
Nov 12, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Miki IgarashiStanley I Rapoport

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Related Papers

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
A Baranczyk-Kuzma, K L Audus
© 2022 Meta ULC. All rights reserved