Demonstration of Actinomyces and Arachnia species in cervicovaginal smears by direct staining with species-specific fluorescent-antibody conjugate.

Journal of Clinical Microbiology
L PineJ M Brown

Abstract

For direct observation of microaerophilic actinomycetes by fluorescent antibody, a procedure was developed in which pepsin treatment and rhodamine conjugate of normal serum were used to reduce nonspecific staining in cervicovaginal smears. Actinomyces israelii, Actinomyces naeslundii, and Arachnia propionica were observed in cervicovaginal smears from women who did use and who did not use an intrauterine contraceptive device. A. israelii was found more commonly in women with an intrauterine contraceptive device, but no evidence was obtained that the use of an intrauterine contraceptive device influenced the presence of either A. propionica or A. naeslundii.

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Citations

Jun 1, 1987·Advances in Contraception : the Official Journal of the Society for the Advancement of Contraception·A G de ClercqG Claeys
Jan 1, 1985·Journal of Clinical Pathology·E Persson, K Holmberg
Feb 1, 1993·Genitourinary Medicine·D T Evans
Mar 13, 2020·International Journal of Dermatology·Katrice M KaranfilianRobert A Schwartz
Nov 1, 1989·The Australian & New Zealand Journal of Obstetrics & Gynaecology·A G Cleghorn, R G Wilkinson
Nov 1, 1990·International Journal of STD & AIDS·J Hare
Jan 1, 1983·Mycopathologia·J B JonesW White
Dec 1, 1984·Journal of Clinical Pathology·R G Mitchell, M R Crow
Feb 6, 1982·British Medical Journal·R Smith
Nov 1, 1984·Journal of Clinical Microbiology·E Persson, K Holmberg

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Actinomycosis is a rare, chronic, and slowly progressive granulomatous disease caused by filamentous Gram positive anaerobic bacteria from the Actinomycetaceae family (genus Actinomyces). The disease is characterised by the formation of painful abscesses in the mouth, lungs,breast or gastrointestinal tract. Discover the latest research on actinomycosis here.