Demonstration of aflatoxin inhibitory activity in a cotton seed coat xylan.

Applied and Environmental Microbiology
J E MellonE Roberts

Abstract

An inhibitor of aflatoxin biosynthesis localized in the seed coats of developing cotton was partially purified and characterized. Aqueous extracts from 25-day postanthesis seed coat tissue inhibited aflatoxin (B(inf1)) production in liquid cultures of Aspergillus flavus AF13. Inhibition was concentration dependent, with a 50% effective dose of 173 (mu)g of crude extract per ml of medium. The inhibitor was neutral in charge. Two active fractions were obtained from crude preparations by gel filtration chromatography (BioGel P-100). The purest fraction eluted in the void volume. Carbohydrate composition analysis of this void volume inhibitor indicated a composition of xylose (>90%) and mannose. Aflatoxin production in vitro was inversely related to inhibitor concentration in the fermentation medium (log of aflatoxin versus log of [inhibitor]; r(sup2) = 0.82; P < 0.002). The void volume inhibitor had a 50% effective dose of 6.2 (mu)g/ml, a 28-fold purification of the inhibitor material. These data support the hypothesis that seed coat inhibitory activity is associated with a cottonseed-specific xylan.

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Nov 21, 1983·Mycopathologia·T P HensarlingA Ciegler
May 11, 1964·Biochimica Et Biophysica Acta·J ADYE, R I MATELES

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Citations

May 20, 2000·Biotechnology & Genetic Engineering Reviews·A Ebringerová, Z Hromádková

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