PMID: 7016720Jan 1, 1981Paper

Demonstration of astrocytes in cultured amniotic fluid cells of three cases with neural-tube defect

Human Genetics
M CremerT Voigtländer

Abstract

We have investigated the origin of rapidly adhering (RA) cells in three cases of neural tube defects (two anencephali, one encephalocele). We were able to demonstrate the presence of glial fibrillary acidic (GFA) protein in variable percentages (4--80%) of RA cells cultured for 4--6 days by use of indirect immunofluorescence with GFA antiserum. Cells cultured from amniotic fluids of normal pregnancies and fetal fibroblasts were completely GFA protein negative. GFA protein is well established as a highly specific marker for astrocytes. Demonstration of astrocytes may prove to be a criterion of high diagnostic value for neural tube defects. The percentage of astrocytes decreased with increasing culture time, while the percentage of fibronectin positive cells increased both in amniotic fluid cell cultures from neural tube defects and normal pregnancies.

References

Jun 1, 1975·Journal of Medical Genetics·G R SutherlandJ B Scrimgeour
Sep 1, 1977·Clinical Obstetrics and Gynecology·M Seppälä
Sep 1, 1978·The Journal of Cell Biology·E CrouchP Bornstein
Nov 24, 1978·Human Genetics·T VoigtländerT M Schroeder
Apr 1, 1977·Experimental Cell Research·L B ChenJ K McDougall
May 7, 1971·Brain Research·L F EngB Gerstl
Mar 28, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·P K BuamahA M Ward
Dec 1, 1981·Journal of Neuroimmunology·J Schnitzer, M Schachner
Jan 1, 1980·American Journal of Obstetrics and Gynecology·S SarkarO W Jones

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Citations

Jan 1, 1982·Human Genetics·P Medina-Gómez, T H Johnston
Mar 1, 2012·Archives of Pharmacal Research·Sunyoung JooSang Jin Lee
May 29, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·N Van RegemorterA Lowenthal
Jan 1, 1984·Human Genetics·H von KoskullI Virtanen
Jan 16, 2008·Journal of Cellular and Molecular Medicine·Akiva J Marcus, Dale Woodbury
Oct 26, 2012·Fundamental & Clinical Pharmacology·Felipe de Lara JanzSergio Paulo Bydlowski
Oct 6, 2012·Organogenesis·Mara Cananzi, Paolo De Coppi
Oct 1, 1983·Prenatal Diagnosis·P Medina-Gomez, J B Bard
Nov 1, 1984·Prenatal Diagnosis·M AlbrechtsenB Nørgaard-Pedersen

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