Demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of Aedes aegypti

PLoS Neglected Tropical Diseases
Irma Sánchez-VargasKen E Olson

Abstract

Aedes aegypti is the primary mosquito vector of dengue viruses (DENV; serotypes 1-4). Human-mosquito transmission cycles maintain DENV during epidemics but questions remain regarding how these viruses survive when human infections and vector abundance are minimal. Aedes mosquitoes can transmit DENV within the vector population through two alternate routes: vertical and venereal transmission (VT and VNT, respectively). We tested the efficiency of VT and VNT in a genetically diverse laboratory (GDLS) strain of Ae. aegypti orally infected with DENV2 (Jamaica 1409). We examined F1 larvae from infected females generated during the first and second gonotrophic cycles (E1 and E2) for viral envelope (E) antigen by amplifying virus in C6/36 cells and then performing an indirect immunofluorescence assay (IFA). RT-PCR/nested PCR analyses confirmed DENV2 RNA in samples positive by IFA. We observed VT of virus to larvae and adult male progeny and VNT of virus to uninfected virgin females after mating with males that had acquired virus by the VT route. We detected no DENV2 in 30 pools (20 larvae/pool) of F1 larvae following the first gonotrophic cycle, suggesting limited virus dissemination at 7 days post-infection. DENV2 was detected by IFA...Continue Reading

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Citations

Apr 30, 2020·Pathogens·Paula Rozo-LopezBarbara S Drolet
Oct 16, 2020·PLoS Neglected Tropical Diseases·Zetian LaiXiao-Guang Chen
May 5, 2021·Vector Borne and Zoonotic Diseases·José I Torres-AvendañoJosé M Zazueta-Moreno
Jun 21, 2021·Virology·Dilip K NagLaura D Kramer
May 15, 2019·Intervirology·Luis A Alonso-PalomaresMa Isabel Salazar
Jan 23, 2022·Journal of Medical Entomology·Stephen A PeinadoLyric C Bartholomay

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PCR

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GraphPad prism

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