Feb 1, 1974

Demonstration of electrogenic Na+-dependent D-glucose transport in intestinal brush border membranes

Proceedings of the National Academy of Sciences of the United States of America
H Murer, U Hopfer


Na(+)-coupled D-glucose transport was studied in isolated membrane vesicles from intestinal brush borders. Concentration gradients of SCN(-), K(+), and H(+) were established between the intravesicular solution and the incubation medium and their influence on D-glucose uptake from the medium was measured. A gradient (medium > vesicle) of NaSCN, but not of KSCN, produced a transient overshoot of D-glucose uptake above the equilibrium level. Similarly, an increase of the membrane conductance with valinomycin (K(+)-conductance) or with uncoupling agents of oxidative phosphorylation (H(+)-conductance) induced an overshooting D-glucose uptake, provided a (vesicle > medium) K(+)-gradient or a H(+)-gradient, respectively, was present in each case. The transient overshoot is evidence that D-glucose was taken up against its concentration gradient (up to 10-fold). The gradients of SCN(-), K(+) (in the presence of valinomycin), and H(+) (in the presence of uncouplers) are thought to contribute to the "driving" force for this "active" D-glucose transport by changing the electrical potential across the vesicle membrane and thus making the inside more negative (with respect to the medium). These experiments, therefore, provide evidence that t...Continue Reading

  • References17
  • Citations132


  • References17
  • Citations132


Mentioned in this Paper

Uncoupling Agents
Resting Potentials
Carbon Radioisotopes
Structure of Intestinal Gland
Anhydrous Dextrose
Epithelial Cells

About this Paper

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