Demonstration of extensive GABA synthesis in the small population of GAD positive neurons in cerebellar cultures by the use of pharmacological tools

Neurochemistry International
Ursula SonnewaldH S Waagepetersen

Abstract

Cultures of dissociated cerebella from 7-day-old mice were maintained in vitro for 1-13 days. GABA biosynthesis and degradation were studied during development in culture and pharmacological agents were used to identify the enzymes involved. The amount of GABA increased, whereas that of glutamate was unchanged during the first 5 days and both decreased thereafter. The presence of aminooxyacetic acid (AOAA, 10 microM) which inhibits transaminases and other pyridoxal phosphate dependent enzymes including GABA-transaminase (GABA-T), in the culture medium caused an increase in the intracellular amount of GABA and a decrease in glutamate. The GABA content was also increased following exposure to the specific GABA-T inhibitor gamma-vinyl GABA. From day 6 in culture (day 4 when cultured in the presence of AOAA) GABA levels in the medium were increased compared to that in medium from 1-day-old cultures. Synthesis of GABA during the first 3 days was demonstrated by the finding that incubation with either [1-(13)C]glucose or [U-(13)C]glutamine led to formation of labeled GABA. Synthesis of GABA after 1 week in culture, when the enzymatic machinery is considered to be at a more differentiated level, was shown by labeling from [U-(13)C]glu...Continue Reading

References

Aug 1, 1989·Neurochemical Research·J Drejer, A Schousboe
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K SaitoJ E Vaughn
Oct 1, 1974·Journal of Neurochemistry·J Y Wu, E Roberts
Oct 1, 1996·Neurochemistry International·L A Borden
Apr 23, 2004·Trends in Neurosciences·Robert T FremeauRobert H Edwards
Nov 5, 2004·Journal of Neurochemistry·Ursula SonnewaldHelle Waagepetersen
Jul 28, 2005·Journal of Neurochemistry·Eiliv BrennerUrsula Sonnewald
Feb 10, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lasse K BakHelle S Waagepetersen
Jan 1, 1985·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·O M LarssonA Schousboe

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Citations

Oct 8, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cristina Suñol
Jan 12, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Niladri BasuLaurie H M Chan
Oct 13, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Elisabeth OlstadUrsula Sonnewald
Sep 12, 2007·Journal of Neuroscience Research·Mary C McKenna
May 6, 2019·Cerebral Cortex·Tayyaba QureshiFarrukh Abbas Chaudhry
Feb 17, 2007·Journal of Neuroscience Research·Elisabeth OlstadUrsula Sonnewald
Feb 22, 2012·Molecular and Cellular Biochemistry·Ethel Antunes WilhelmCristina Wayne Nogueira

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