Demonstration of functionally different interactions between phospholipase C-gamma and the two types of platelet-derived growth factor receptors.

The Journal of Biological Chemistry
A ErikssonL Claesson-Welsh

Abstract

Phosphorylated tyrosine residues in receptor tyrosine kinases serve as binding sites for signal transduction molecules. We have identified two autophosphorylation sites, Tyr-988 and Tyr-1018, in the platelet-derived growth factor (PDGF) alpha-receptor carboxyl-terminal tail, which are involved in binding of phospholipase C-gamma (PLC-gamma). The capacities of the Y988F and Y1018F mutant PDGF alpha-receptors, expressed in porcine aortic endothelial cells, to bind PLC-gamma are 60 and 5% of that of the wild-type receptor, respectively. Phosphorylated but not unphosphorylated peptides containing Tyr-1018 are able to compete with the intact receptor for binding to immobilized PLC-gamma SH2 domains; a phosphorylated Tyr-988 peptide competes 10 times less efficiently. The complex between PLC-gamma and the PDGF alpha-receptor is more stable than that of PLC-gamma and the PDGF beta-receptor. However, PDGF stimulation results in a smaller fraction of tyrosine-phosphorylated PLC-gamma and a smaller accumulation of inositol trisphosphate in cells expressing the alpha-receptor as compared with cells expressing the beta-receptor. We conclude that phosphorylated Tyr-988 and Tyr-1018 in the PDGF alpha-receptor carboxyl-terminal tail bind PLC-...Continue Reading

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Citations

Feb 13, 2001·Biochemical and Biophysical Research Communications·M LuchtefeldB Schieffer
Apr 1, 1996·The International Journal of Biochemistry & Cell Biology·L Claesson-Welsh
Sep 18, 1998·Biochimica Et Biophysica Acta·C H HeldinL Rönnstrand
Oct 1, 1996·General Pharmacology·A D HughesC Demoliou-Mason
May 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Melanie Van StryKaren Symes
Apr 16, 1998·The Journal of Biological Chemistry·J A GelderloosA Kazlauskas
Nov 29, 1996·The Journal of Biological Chemistry·K YokoteL Claesson-Welsh
Apr 29, 1998·The Journal of Biological Chemistry·R C VenemaM B Marrero
Nov 14, 1998·The American Journal of Physiology·M B MarreroR C Venema
Jun 23, 2004·Cytokine & Growth Factor Reviews·Michelle Tallquist, Andrius Kazlauskas
Jun 17, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sandra VerstraelenRosette Van Den Heuvel
Feb 24, 2015·Gene Expression·Alexander Kikuchi, Satdarshan Pal Monga

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