Apr 1, 1973

Demonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts

Proceedings of the National Academy of Sciences of the United States of America
T ImaizumiK Scherrer


Highly purified globin mRNA from ducks was copied with RNA-directed DNA polymerase from avian myeloblastosis virus into anti-messenger DNA. With excess RNA, more than 90% of this DNA annealed back to its template with a C(o)t/2 value of 7.5 x 10(-4) mol.sec. liter(-1); the melting temperature of the hybrid was 86 degrees . Giant nuclear RNA fractions with sedimentation coefficients of more than 50 S formed hybrids of almost equal stability at C(o)t/2 values of 0.05-0.42 mol.sec. liter(-1), indicating amRNA content of 0.3-1.5%. 12S RNA from the same polyribosomes and nuclear giant RNA from HeLa cells did not cross-hybridize. Although a large part of the giant RNA broke down in 99% dimethylsulfoxide gradients, RNA fractions sedimenting faster than 28S rRNA still were found to consist of up to 0.03% globin mRNA sequences. Thus, the mRNA sequences are contained in the covalent structure of giant nuclear precursors, which are termed precursor-mRNA.

Mentioned in this Paper

Centrifugation, Density Gradient
DNA, Viral
Rous-Associated Virus
Genetic Template
Genomic Hybridization
Poly(A) Tail
RNA-Directed DNA Polymerase

About this Paper

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