Demonstration of granzyme A and perforin messenger RNA in the synovium of patients with rheumatoid arthritis

Arthritis and Rheumatism
U Müller-LadnerS Gay

Abstract

To examine the gene expression of 2 highly specific markers of cytotoxic T lymphocyte (CTL) activation, the serine protease granzyme A and the pore-forming protein perforin, in synovial tissue of patients with rheumatoid arthritis (RA), and to compare the findings with those in osteoarthritis (OA) synovial tissue. Snap-frozen synovial tissue specimens from 9 patients with RA and 5 patients with OA were examined. The number of CTL that expressed granzyme A or perforin messenger RNA was determined by in situ hybridization using nonradioactive riboprobes for granzyme A and perforin, and by a novel in situ reverse transcriptase technique. The signals were visualized by an immunogold-silver immunohistochemistry technique and compared with immunohistochemical labeling of T and B cells. Additional double-labeling was achieved using anti-type IV collagen, anti-macrophage (anti-CD68), anti-T lymphocyte (anti-CD45RO), anti-B lymphocyte (anti-CD20), and anti-natural killer cell (anti-CD56) antibodies in an alkaline phosphatase-anti-alkaline phosphatase assay. Granzyme A and perforin messenger RNA (mRNA) was observed in CTL in synovial specimens from all of the RA patients, whereas in specimens from OA patients only a few, single cells wit...Continue Reading

References

Jul 5, 1979·The New England Journal of Medicine·J J HooksA L Notkins
Dec 1, 1992·Clinical Rheumatology·D C NordströmJ Tschopp
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G M GriffithsI L Weissman
Jun 1, 1989·The Journal of Experimental Medicine·L H YoungJ D Young
Jan 1, 1989·Current Topics in Microbiology and Immunology·E R PodackM Lichtenheld
Jan 1, 1989·Current Topics in Microbiology and Immunology·D E Jenne, J Tschopp
Apr 19, 1988·Biochemistry·J Tschopp, C V Jongeneel
Oct 1, 1988·British Journal of Rheumatology·S RuschenH Warnatz
Jan 1, 1985·Annual Review of Immunology·P A Henkart
Dec 1, 1993·British Journal of Rheumatology·G StranskyS Gay

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Citations

Dec 31, 1998·Arthritis and Rheumatism·T NamekawaC M Weyand
Mar 1, 1997·Arthritis and Rheumatism·T HasunumaK Nishioka
Aug 13, 2013·Lasers in Medical Science·Fernando Pereira CarlosStella Regina Zamuner
Dec 1, 1996·Journal of the Neurological Sciences·U Müller-LadnerS R Barnum
Jul 1, 2005·Arthritis Research & Therapy·Kristin BauerSaleh M Ibrahim
Nov 30, 2000·Arthritis Research·P P Tak, F C Breedveld
Feb 26, 1999·International Reviews of Immunology·U Müller-LadnerS Gay
Oct 6, 1998·Cell Adhesion and Communication·G H HersmannR Bräuer
May 4, 2011·Autoimmunity Reviews·Paola ConigliaroRoberto Perricone
Apr 5, 2013·BioMed Research International·Yubo SunHelen E Gruber
Mar 29, 2006·Rheumatology·L C HuberT Pap
Jun 9, 2020·Frontiers in Immunology·Kim R van DaalenNiels Bovenschen
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·U Müller-LadnerS Gay

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