Demonstration of GTP-binding proteins and ADP-ribosylated proteins in rat liver Golgi fraction

Biochemical and Biophysical Research Communications
C TokiY Ikehara

Abstract

A Golgi-rich fraction isolated from rat liver was found to contain GTP-binding proteins with 20-25 kDa, which were tightly bound to the Golgi membrane. The Golgi fraction also contained two species of proteins which were ADP-ribosylated by bacterial toxins. Protein(s) which was ADP-ribosylated by botulinum toxin had a similar molecular mass as those with GTP-binding activity but was easily released from the membrane. Another protein with 46 kDa which was ADP-ribosylated by pertussis toxin was tightly bound to the membrane but had no significant GTP-binding activity under conditions tested here. These proteins were much less or negligible in the plasma membrane and the endoplasmic reticulum.

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Citations

Nov 1, 1992·The Journal of Cell Biology·P AdamsonA Hall
Sep 14, 1990·Biochemical and Biophysical Research Communications·J R DidsburyR Snyderman
Jun 15, 1990·Biochemical and Biophysical Research Communications·T OhmoriY Takai

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