PMID: 108182Mar 1, 1979

Demonstration of human apolipoprotien A in isolated mucosal cells from small intestine and isolated hepatocytes

Gut
U HopfA Capurso

Abstract

Isolated mucosal cells from the human jejunum and stomach, cryostat sections from the jejunum, isolated parenchymal liver cells and lymphocytes were investigated for the presence of apolipoprotien A (apoA). Antisera against purified human apoA-I and apoA-II were raised in rabbits and conjugated with fluorescein-isothiocyanate (FITC). Mucosal cells from jejunum and stomach were isolated with pronase from tissue obtained from operated patients. ApoA-I and apoA-II could be demonstrated in isolated mucosal cells as well as in cryostat sections from the jejunum. The fluorescence pattern in isolated jejunal cells was coarse granular. In the radial gel diffusion test the homogenate from mucosal cells of jejunum showed a single precipitation line with anti-apoA-I and with anti-apoA-II, respectively. The reaction was more intensive with anti-apoA-I than with anti-apoA-II. Isolated gastric cells were negative for apoA. Hepatocytes incubated with FITC anti-apoA-I showed a fine granular fluorescence pattern in the cytoplasm. Anti-apoA-II did not react with hepatocytes. There was no evidence for an in vivo fixation of serum-apoA at the surface of isolated mucosal cells from jejunum or isolated hepatocytes. The results support the hypotheses...Continue Reading

References

Sep 1, 1976·The Journal of Clinical Investigation·R L HamiltonR J Havel
Mar 1, 1977·The Journal of Clinical Investigation·G AssmannK Oette
Jul 1, 1977·The Journal of Clinical Investigation·M C Cheung, J J Albers
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R M Glickman, P H Green
Feb 1, 1978·The Journal of Clinical Investigation·P H GreenR M Glickman
Apr 1, 1978·Atherosclerosis·A Van TolF Vlaspolder
Jan 30, 1978·Biochemical and Biophysical Research Communications·E J SchaeferH B Brewer
Mar 11, 1976·The New England Journal of Medicine·U HopfW Arnold
Mar 1, 1976·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S J LanE C Schreiber
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W Arnold, H von Mayersbach
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Mar 1, 1966·The Journal of Clinical Investigation·P S RoheimH A Eder

Citations

Apr 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·C H Florén, A Gustafson
May 1, 1987·Scandinavian Journal of Gastroenterology·C H FlorénJ J Albers

Related Concepts

Apolipoproteins
Immunofluorescence Assay
Structure of Pyloric Gland
Immunodiffusion Measurement
Structure of Intestinal Gland
Jejunum
Liver
Lymphoid Cells

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