PMID: 2507640Sep 1, 1989Paper

Demonstration of IL-1 alpha, and IL-1 beta secretion by the monocytic leukemia cell line, THP-1

Journal of Immunological Methods
E V GaffneyL A Wagner

Abstract

This study examined the secretion of IL-1 alpha and IL-1 beta by THP-1 leukemia cells following activation with mezerein and promotion of synthesis by interferon (IFN-gamma). Interleukin-1 (IL-1) was not detected by co-mitogenic thymocyte assays of crude supernates. Isoelectrofocusing of concentrated medium showed that all biologically active IL-1 migrated at a pH of 6.8-7.2, indicating that the major secreted form was IL-1 beta. Double antibody ELISA confirmed the presence of IL-1 beta, but failed to detect IL-1 alpha in isofocused fractions. Although it appeared that THP-1 cells do not secrete IL-1 alpha; an inhibitor of thymocyte response to IL-1 was present in conditioned medium, migrated in an acidic pH range and masked the expression of biologically active rIL-1 alpha and rIL-1 beta. In contrast, IL-1 alpha was detected using a cell blotting assay. This technique permitted visualization of subpicogram levels of IL-1 when secreted by cells attached to an immunoblotting paper. Cell blotting showed that a greater proportion of attached cells incubated for 24 h in medium containing mezerein and IFN-gamma secreted IL-1 than cells in control medium. In conclusion, the amount of immunoreactive or biologically active IL-1 alpha s...Continue Reading

References

Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R C FuhlbriggeD D Chaplin
Oct 31, 1988·Biochemical and Biophysical Research Communications·M TurnerM Feldmann
May 1, 1987·Annales De L'Institut Pasteur. Immunology·P E Auron, A C Webb
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J W ChiaoK Leung
Aug 3, 1987·Journal of Immunological Methods·E V GaffneyJ Piscitelli
Feb 1, 1986·Immunology Today·J J OppenheimS K Durum

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Citations

Feb 1, 1991·Immunological Reviews·E V GaffneyG A Koch
Jan 1, 1991·Annals of the New York Academy of Sciences·P RalphA Sampson-Johannes
Dec 1, 1995·Leukemia & Lymphoma·H T Hassan, H G Drexler
Apr 28, 2006·Evolution; International Journal of Organic Evolution·Carol GoodwillieKermit Ritland
Mar 29, 2005·Evolution; International Journal of Organic Evolution·Emmanuelle Porcher, Russell Lande

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