PMID: 8581881Dec 1, 1995Paper

Demonstration of isoforms of the estrogen receptor in the bone tissues and in osteoblastic cells

Calcified Tissue International
S HoshinoH Orimo

Abstract

Expression of isoforms of estrogen receptor (ER) was examined in the bone tissues. Reverse transcriptase-polymerase chain reaction ((RT-PCR) using specific primers for rat ER cDNA was performed with total RNA from rat bone tissues. Then, we sequenced the amplified products after cloning and identified two isoforms of the ER and the wild-type ER. One of the ER mRNA isoforms did not have the region corresponding to exon 4 and the other isoform did not have the region corresponding to both exon 3 and exon 4. These isoforms were designated as ER delta 4 isoform and ER delta 3/4 isoform, respectively. The existence of these isoforms was also confirmed by ROS-17/2.8 osteoblastic osteosarcoma cells. Chloramphenicol acetyltransferase assay showed that these isoforms lost estrogen dependent transactivation activities. We suggest that the ER isoforms may play some roles in the bone metabolism in which estrogen is essential to maintain bone density.

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Citations

Jun 16, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Barbara CardazzoLuca Bargelloni
Oct 17, 2003·Contraception·Susan D ReedSusan M Ott
Jan 12, 2000·Molecular and Cellular Endocrinology·B LuL C Murphy
Aug 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·L C MurphyP H Watson
May 28, 1997·Annals of the New York Academy of Sciences·D K Katzman, R B Zipursky
Apr 21, 2018·Npj Regenerative Medicine·Hareklea MarkidesAlicia J El Haj
Jun 8, 2004·Endocrine Reviews·Dirk VanderschuerenClaes Ohlsson

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