PMID: 51802Jan 1, 1975Paper

Demonstration of membrane-bound immunoglobulin determinants and the significance of a quantitative characterization of the antisera used

Folia Biologica
F EmmrichH Schwenke

Abstract

Lymphocytes from 22 healthy blood donors were tested for the presence of immunoglobulin determinants on their surfaces by indirect membrane immunofluorescence. The proportion of MemIF+ cells was, on the average, 8.7% (3-18) for IgM and 15.8% (8-28) for IgG. The small differences in the proportion of MemIF+ lymphocytes upon use of various anti-IgM sera could be attributed mainly to the different antibody concentrations. The importance of a quantitative characterization of the antisera used was pointed out.

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