Demonstration of neuron-glia transfer of precursors for GABA biosynthesis in a co-culture system of dissociated mouse cerebral cortex.

Neurochemical Research
Renata LekeHelle S Waagepetersen

Abstract

Co-cultures of neurons and astrocytes were prepared from dissociated embryonic mouse cerebral cortex and cultured for 7 days. To investigate if these cultures may serve as a functional model system to study neuron-glia interaction with regard to GABA biosynthesis, the cells were incubated either in media containing [U-(13)C]glutamine (0.1, 0.3 and 0.5 mM) or 1 mM acetate plus 2.5 mM glucose plus 1 mM lactate. In the latter case one of the 3 substrates was uniformly (13)C labeled. Cellular contents and (13)C labeling of glutamate, GABA, aspartate and glutamine were determined in the cells after an incubation period of 2.5 h. The GABA biosynthetic machinery exhibited the expected complexity with regard to metabolic compartmentation and involvement of TCA cycle activity as seen in other culture systems containing GABAergic neurons. Metabolism of acetate clearly demonstrated glial synthesis of glutamine and its transfer to the neuronal compartment. It is concluded that this co-culture system serves as a reliable model in which functional and pharmacological aspects of GABA biosynthesis can be investigated.

References

Jan 1, 1993·Developmental Neuroscience·A SchousboeL Hertz
Jan 15, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H S WaagepetersenA Schousboe
Mar 25, 2000·Neurochemistry International·H S WaagepetersenA Schousboe
Jul 23, 2003·Current Drug Targets. CNS and Neurological Disorders·Alan SarupArne Schousboe
Nov 6, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anne-Karine Bouzier-SoreLuc Pellerin
Feb 10, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lasse K BakHelle S Waagepetersen

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Citations

Nov 1, 2011·Neurotoxicity Research·Cleanthe Spanaki, Andreas Plaitakis
May 7, 2009·Journal of Neurochemistry·Helle M SickmannHelle S Waagepetersen
May 16, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sherry DadsetanHelle S Waagepetersen
Aug 29, 2012·Neurochemical Research·Sofie C LangeMichael D Norenberg
Jul 29, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Rossana OcchipintiDaniela Calvetti
Apr 5, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Daniela Calvetti, Erkki Somersalo

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