PMID: 6104544Mar 31, 1980Paper

Demonstration of neuronal activities in primary cultures from fetal mouse hypothalamus maintained in a serum-free medium

Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles
A Faivre-BaumanA Tixier-Vidal

Abstract

Fetal neuronal cells dissociated from mouse hypothalamus are able to grow in a serum-free medium for two to four weeks. Several neuronal activities have been measured during the in vitro cell development. Whereas glutamic acid decarboxylase (EC 4.1.1.15) activity disappears, those of thyroliberin (TRH) and tyrosine hydroxylase (EC. 1.14.16.2) remain stable, and acetylcholine transferase one (EC 2.3.1.6) rises earlier than in a serum supplemented medium. These results show that primary cultures grown in serum-free medium offer a promising model for studying neuronal activity differentiation.

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