PMID: 8959530Nov 1, 1996Paper

Demonstration of noradrenaline-immunoreactive nerve fibres in the liver

The Journal of International Medical Research
Y FukudaT Hayakawa

Abstract

To demonstrate noradrenaline-immunoreactive nerve fibres in liver tissues, we used an antibody to noradrenaline in the immunostaining of liver tissues from rats, guinea-pigs and humans. The tissue specimens were fixed by perfusion or immersion with cacodylate buffer containing sodium metabisulphate and glutaraldehyde, and cryostat sections were prepared. An indirect peroxidase-labelled antibody method was used for staining noradrenaline. Noradrenaline-immunoreactive nerve fibres were localized around blood vessels in the portal area and around the central vein. There were differences between the species in the intralobular distribution of noradrenaline-immunoreactive fibres. Normal guinea-pig and human liver showed intralobular noradrenaline-immunoreactive fibres while rat liver did not. Noradrenaline-immunoreactive fibres were absent from regenerating nodules in a human cirrhotic liver. This method of demonstrating noradrenaline directly using perfusion- or immersion-fixation is appropriate for studying innervation in normal and damaged livers of various species including humans.

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Citations

Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Gerhard P Püschel
May 13, 2010·Acta Veterinaria Hungarica·Eva PongorErzsébet Fehér
Feb 15, 2019·Biology·Magdalena BlaszkiewiczKristy L Townsend
Jun 9, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kei Mizuno, Yoshiyuki Ueno
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Liliane DubuissonJean Rosenbaum
Jan 14, 1998·The Journal of International Medical Research·Y FukudaT Hayakawa
Jul 14, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bess M MillerWolfram Goessling
Jan 12, 2010·Biochimica Et Biophysica Acta·Chun-Xia YiAndries Kalsbeek

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