Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)

Biochemical and Biophysical Research Communications
T NishidaM Takigawa

Abstract

The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8. The binding of 125I-labeled recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Kd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of 125I-CTGF-receptor complex with an apparent molecular weight of 280 kDa. The 125I-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF. In addition, the 125I-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells.

References

Jul 1, 1995·The Journal of Investigative Dermatology·K KikuchiK Takehara
Apr 1, 1994·Japanese Journal of Cancer Research : Gann·J D ZhuM Takigawa
Aug 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·B S Oemar, T F Lüscher

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Citations

Mar 14, 2002·The American Journal of Pathology·Maria Cristina ManaraKatia Scotlandi
May 28, 2010·Expert Reviews in Molecular Medicine·Wendy C Burns, Merlin C Thomas
Jan 13, 2006·Clinical and Experimental Pharmacology & Physiology·Janette K Burgess
Nov 10, 2012·Investigative Ophthalmology & Visual Science·Paulette M RobinsonGregory S Schultz
Nov 10, 2012·Investigative Ophthalmology & Visual Science·Siva S RadhakrishnanGregory S Schultz
Aug 27, 2011·Molecular Endocrinology·Takashi NagashimaMartin M Matzuk
Oct 5, 2011·Critical Reviews in Eukaryotic Gene Expression·John A ArnottSteven N Popoff
Mar 21, 2009·The Biochemical Journal·Eriko AoyamaMasaharu Takigawa
Sep 29, 2004·Osteoarthritis and Cartilage·Shunsuke OmotoMasaharu Takigawa
Apr 3, 2007·Biochemical and Biophysical Research Communications·Mitsuaki OnoTakuo Kuboki
Oct 28, 2017·Journal of Cell Communication and Signaling·Masaharu Takigawa
Apr 20, 2004·Current Opinion in Nephrology and Hypertension·Nadia Abdel Wahab, Roger M Mason
May 13, 2008·Cell Transplantation·Mitsuaki OnoTakuo Kuboki
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V ParadisP Bedossa
Jun 25, 2013·Journal of Cell Communication and Signaling·Masaharu Takigawa
Aug 20, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takashi NishidaMasaharu Takigawa
Mar 14, 2002·Current Rheumatology Reports·Andrew LeaskDavid J Abraham
Sep 12, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Olav A Gressner, Axel M Gressner
Dec 17, 2004·Journal of the American Society of Nephrology : JASN·Nadia Abdel WahabRoger M Mason
Aug 21, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J K CreanF Martin
Dec 22, 1999·The Journal of Biological Chemistry·K HishikawaT Fujii
Apr 22, 2016·Journal of Cell Communication and Signaling·Lester F Lau
Sep 15, 2011·Endocrinology·Takashi NishidaMasaharu Takigawa
Dec 22, 1999·Endocrine Reviews·V HwaR G Rosenfeld

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