Demonstration of renin gene expression in nephroblastoma by in situ hybridization

The Journal of Pathology
K J McKenzieG B Lindop

Abstract

Most patients with nephroblastoma have high levels of plasma renin and some are hypertensive. Blood pressure falls after removal of the affected kidney, suggesting that nephroblastoma is associated with renin production either by the tumour or by the kidney. In this study, direct evidence was sought of renin gene expression in nephroblastoma using in situ hybridization. Digoxigenin-labelled riboprobes and an immunoperoxidase technique were used to detect cells containing renin mRNA: this showed renin gene expression in 9 out of 12 cases. There were positive cells within metanephric blastema and in occasional neoplastic glomeruloid structures, confirming that in seven cases nephroblastoma tumour cells expressed the renin gene. However, renin gene expression was also demonstrated in perivascular cells of uncertain lineage in seven cases; in five cases there was evidence of renin gene expression in both tumour cells and perivascular cells. The latter finding raises the possibility that some of the cells expressing the renin gene could be stromal cells. It is concluded that nephroblastomas contain cells that express the renin gene and that some are tumour cells, while other perivascular cells may be stromal cells.

References

Jun 1, 1978·The Journal of Pediatrics·K J ShethT A Good
Jun 1, 1990·The Journal of Pathology·G B LindopG D Birnie
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·G B LindopB J Leckie
May 1, 1988·Journal of Pediatric Surgery·K YokomoriY Tsuchida
Mar 1, 1987·Journal of Pediatric Surgery·R CarachiB J Leckie
Jun 1, 1970·Archives of Disease in Childhood·J D MitchellD A McCredie
Jul 1, 1984·Journal of Clinical Pathology·G B LindopA A Gibson
Jan 1, 1984·Journal of Clinical Pathology·G B Lindop, S Fleming
Dec 1, 1984·DNA·J A HardmanJ Shine

❮ Previous
Next ❯

Citations

May 13, 1998·Journal of Clinical Pathology·M R Stephen, G B Lindop
May 23, 2008·Kidney International·Andreas SteegeRalf Mrowka
Jun 14, 2006·Pediatric Radiology·Douglas ByerlyFrederick Ruymann
Jun 24, 2006·Pediatric Blood & Cancer·M H MaasM M van den Heuvel-Eibrink
Jan 20, 2006·Pediatric Surgery International·G P Hadley, M Mars

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Pediatric Surgery
M A JohnstonB J Leckie
Clinical and Experimental Hypertension. Part A, Theory and Practice
G B LindopB J Leckie
Journal of Pediatric Surgery
R CarachiB J Leckie
© 2021 Meta ULC. All rights reserved