Demonstration of reverse fatty acid transport from rat cardiomyocytes.

Journal of Lipid Research
Byung-Hyun ParkRoger H Unger

Abstract

Fatty acids flow from adipocytes to nonadipose tissues during fasting and exercise and normally are fully oxidized. To determine if nonadipose tissues can export unoxidized FA when FA influx exceeds oxidation, neonatal cardiomyocytes were cultured in 1 microCi (14)C-palmitate in the presence of etomoxir to block oxidation. The cells took up and stored 25% of the radioactivity as (14)C-triacylglycerol in 12 h, but 4.5% of the label was released in 3 h and comigrated with (14)C-palmitate. Both uptake and release of radioactivity were increased by insulin and reduced by the nonspecific inhibitors of FA transporters phloretin and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Perfused hearts from etomoxir-treated lean rats released 221 +/- 59 nmol/10 min of FA. Hearts from high-fat-fed lean rats released 366 +/- 172 nmol/10 min (P < 0.05). Hearts from obese rats released 744 +/- 260 and 1,578 +/- 630 nmol/10 min at 8 and 12 weeks of age, respectively. Perfusion with insulin increased FA release by 32%. In vitro and ex vivo findings suggest that nonadipose tissues such as myocardium can export FA when the unoxidized lipid content is excessive.

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Citations

Dec 14, 2004·Lipids in Health and Disease·Florence GondretJean-François Hocquette
May 13, 2009·The International Journal of Biochemistry & Cell Biology·Jiahua YuByung-Hyun Park
Apr 3, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jiahua YuByung-Hyun Park
May 21, 2005·Biochimica Et Biophysica Acta·Andrew N Carley, David L Severson
Apr 14, 2006·The Journal of Biological Chemistry·Kristin R WildsmithDavid A Ford

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