PMID: 8950282Jan 1, 1996Paper

Demonstration of ribonuclease activity in the plant ribosome-inactivating proteins alpha- and beta-momorcharins

Life Sciences
J W MockW P Fong

Abstract

Alpha- and beta-momorcharins, ribosome-inactivating proteins from Momordica charantia seeds, were utilized in this investigation. Ribonucleolytic cleavage was observed after naked rRNA was incubated with either momorcharin. Beta-momorcharin, and to a lesser extent alpha-momorcharin, also acted on tRNA to release acid-soluble UV-absorbing products. Such activity was optimal at pH around 5.5. Using polyhomoribonucleotides as substrate, it was found that the momorcharins preferentially acted on polyU, but exerted negligible effects on polyA, polyC and polyG. Chromatographic analysis of the reaction product indicated that mono and/or oligo-ribonucleotides, but not free base, were generated from polyU, suggesting that the enzymatic action involved ribonucleolytic cleavage. Similar to the results obtained with tRNA as substrate, beta-momorcharin was about 15-fold more active than alpha-momorcharin on polyU.

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Citations

Jul 3, 2002·Journal of Peptide Science : an Official Publication of the European Peptide Society·X Y Ye, T B Ng
Dec 1, 2001·Biochemical and Biophysical Research Communications·Y W LamH X Wang
Mar 18, 2006·Applied Microbiology and Biotechnology·Hexiang Wang, T B Ng
Dec 4, 2003·Biochemical and Biophysical Research Communications·Hexiang Wang, Tzi Bun Ng
Jan 9, 2004·Protein Expression and Purification·H X Wang, T B Ng
Oct 26, 1999·Life Sciences·P ValbonesiF Stirpe
Mar 29, 2000·The International Journal of Biochemistry & Cell Biology·W P FongT B Ng
Feb 24, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H X Wang, T B Ng
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Kirsten Nielsen, Rebecca S Boston
Nov 10, 2011·Toxins·Assaf Shapira, Itai Benhar
Aug 28, 2007·Biochemical and Biophysical Research Communications·Seong-Cheol ParkKyung-Soo Hahm
Jun 11, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·L XiaT B Ng
Dec 17, 2011·Journal of Ethnopharmacology·Yao MengFubing Shen
Mar 17, 2006·Biochemical and Biophysical Research Communications·H X Wang, T B Ng
Mar 13, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·X Y Ye, T B Ng
Oct 14, 2011·Journal of Separation Science·Xingchuan YaoFubing Shen
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W J PeumansE J Van Damme
Jun 15, 2011·Molecular Biology Reports·Ok Ran LeeDeok-Chun Yang
Jul 13, 2000·Biochemical and Biophysical Research Communications·X Y Ye, T B Ng
Apr 10, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·G ZhangT B Ng

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