Demonstration of specific plasma protein binding sites for somatomedin

The Journal of Clinical Endocrinology and Metabolism
R L Hintz, F Liu


Native somatomedin (SM) has an apparent mol wt of at least 60,000 daltons, while all the purified SM peptides have mol wt 5-8,000 daltsons. We have studied the behavior of somatomedin in plasma during gel filtration at pH 8.1 and pH 2.8, and by the recombination of fractions. Using porcine bioassay for SM, displacement of 125I-insulin by SM, and binding of 125I-SM to plasma proteins, we can demonstrate that the disparity in apparent mol wt of SM is due to reversible binding to plasma proteins which are themselves biologically inactive. This binding site is specific for the SM peptides, saturable, and has high affinity and relatively low capacity.


Feb 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G L WongS J Brandes
Feb 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M M HurleyY Hakeda
Jan 1, 1993·Journal of Cellular Physiology·W R TaylorR W Alexander
Jun 16, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·R KoistinenM Seppälä
Mar 15, 1980·Developmental Biology·A J D'ErcoleL E Underwood
May 1, 1995·Journal of the Neurological Sciences·B W FestoffJ Y Ma
Apr 1, 1986·Metabolism: Clinical and Experimental·T J Merimee
Nov 1, 1979·Molecular and Cellular Endocrinology·R C FranklinA C Herington
Feb 1, 1984·Molecular and Cellular Endocrinology·G L Smith
Jan 1, 1987·Domestic Animal Endocrinology·F C BuonomoD R Campion
Jan 1, 1989·Progress in Growth Factor Research·R C Baxter, J L Martin
Mar 1, 1995·Trends in Endocrinology and Metabolism : TEM·J Zapf
Nov 26, 1986·Biochemical and Biophysical Research Communications·M L HuhtalaM Seppälä
Nov 1, 1988·Baillière's Clinical Endocrinology and Metabolism·C T Roberts, D Leroith
Mar 1, 1997·Cytokine & Growth Factor Reviews·D R Clemmons
Mar 27, 1998·Breast Cancer Research and Treatment·Y Oh
Nov 22, 1979·The New England Journal of Medicine·D R ClemmonsL E Underwood
Feb 14, 1980·The New England Journal of Medicine·L S Phillips, R Vassilopoulou-Sellin
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D J Knauer, G L Smith
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A C MosesO Z Higa
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A J D'ErcoleL E Underwood
Jan 1, 1987·Preparative Biochemistry·B R Glick, M Michalak
May 1, 1990·Clinical Endocrinology·R KoistinenM Seppälä
Jun 15, 1992·European Journal of Biochemistry·K AxelssonP Gellerfors
Aug 1, 1995·Alcoholism, Clinical and Experimental Research·C R Breese, W E Sonntag
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·A SkottnerL Fryklund
Apr 1, 1994·Acta Paediatrica. Supplement·R G RosenfeldY Oh
Jun 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A HöögU G Falkmer
Mar 1, 1985·Acta Physiologica Scandinavica·P G BurholR Jorde
Oct 19, 2006·Neuroendocrinology·Jeff Holly, Claire Perks
Dec 1, 1978·The Journal of Clinical Investigation·J M ConlonR H Unger
Dec 1, 1986·The Journal of Clinical Investigation·R C Baxter, J L Martin
May 1, 1990·The Journal of Clinical Investigation·E T ShapiroH S Tager
Sep 1, 1994·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·S HalimiM Korsić
Apr 1, 1989·Journal of Endocrinological Investigation·A Barreca, F Minuto
Oct 1, 1987·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D R PowellR L Hintz
Mar 1, 1991·Endocrinology and Metabolism Clinics of North America·L N Parker
Dec 1, 1990·American Journal of Obstetrics and Gynecology·R N TaylorJ M Roberts
Sep 1, 1992·Kidney International·A M SuikkariR Koistinen
Sep 1, 1995·Metabolism: Clinical and Experimental·M JeevanandamS R Petersen
Jan 1, 1995·Progress in Growth Factor Research·A BarrecaF Minuto
Jan 1, 1995·Progress in Growth Factor Research·R L Hintz
Aug 1, 1993·Metabolism: Clinical and Experimental·M C GelatoA Monheit
Jul 1, 1989·American Journal of Reproductive Immunology : AJRI·E Y Adashi
Oct 1, 1982·Clinical Endocrinology·J A WassJ J Van Wyk
Mar 1, 1984·Clinics in Endocrinology and Metabolism·D R Clemmons, J J Van Wyk
Mar 1, 1984·Clinics in Endocrinology and Metabolism·J ZapfE R Froesch
Mar 1, 1984·Clinics in Endocrinology and Metabolism·R L Hintz
Jun 1, 1996·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·S M Feld, R Hirschberg
Apr 14, 2015·BioMed Research International·Varsha P BrahmkhatriHanudatta S Atreya
Mar 1, 1991·Baillière's Clinical Obstetrics and Gynaecology·M SeppäläM Julkunen
Mar 31, 1981·Biochemical and Biophysical Research Communications·M H HeulinP Nabet
Dec 20, 2017·Journal of Molecular Endocrinology·L A Bach
Feb 1, 1987·Journal of Dairy Science·P D GluckmanS R Davis
Jun 1, 1989·Journal of Neuroendocrinology·E S GearyA R Hoffman
Jan 1, 1991·Annals of the New York Academy of Sciences·A M SuikkariM Seppälä
Jan 1, 1991·Annals of the New York Academy of Sciences·L C GiudiceJ C Irwin
Feb 14, 1990·European Journal of Biochemistry·R M Lyons, H L Moses
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·D J KnauerG L Smith
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·W F Blum, M B Ranke
Jan 1, 1990·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D T Graves, D L Cochran
Apr 9, 1987·The New England Journal of Medicine·T J MerimeeL L Cavalli-Sforza
Jun 1, 1986·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·B PfeifleH Ditschuneit
Jun 15, 2010·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Sharon M CooleyPeter C Hindmarsh
Oct 1, 1992·Journal of Endocrinological Investigation·G GiordanoF Minuto
Jul 1, 1991·The British Journal of Nutrition·T UmezawaT Noguchi
May 1, 1996·Annals of Clinical Biochemistry·B H MasonI M Holdaway

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