PMID: 8443694Feb 1, 1993Paper

Demonstration of TGF-beta 1 mRNA by in situ hybridization in normal human fracture healing

Calcified Tissue International
J G AndrewD R Marsh

Abstract

The role of transforming growth factor beta (TGF-beta) in fracture healing has previously been investigated in a rodent model, but not in human material. We investigated TGF-beta 1 gene expression in specimens of callus from normally healing human fractures, using in situ hybridization to a cDNA TGF-beta 1 probe and an autoradiographic disclosure system. TGF-beta 1 mRNA was present in areas of proliferation of mesenchymal tissue, bone, and cartilage. Levels of expression were lower in cells in the fracture hematoma and in differentiated (hypertrophic) chondrocytes. These results are compatible with those found in various animal models using immunohistochemistry and support the view that locally produced TGF-beta 1 is a regulator of fracture repair in humans from the early (mesenchymal proliferation) stage to the stage of remodeling of woven bone. They also indicate that, for TGF-beta 1, animal models accurately reflect human bone repair.

References

Nov 1, 1991·Bone and Mineral·J A HoylandA J Freemont
Sep 1, 1991·Developmental Biology·D J Whitby, M W Ferguson
Jan 1, 1989·Connective Tissue Research·M CentrellaE Canalis
Feb 1, 1987·The Journal of Clinical Investigation·C F Nathan
May 1, 1987·Cell and Tissue Research·G JundtA Schulz
Oct 1, 1966·The Biochemical Journal·R A Jeffs, D H Northcote

❮ Previous
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Citations

Jul 9, 1999·Journal of Biomedical Materials Research·F BlanquaertJ P Caruelle
Sep 5, 2002·Journal of Biomedical Materials Research·C LilliP Locci
Aug 1, 1993·Calcified Tissue International·J G AndrewD Marsh
Feb 15, 2001·Bulletin of Experimental Biology and Medicine·N P Omel'yanenkoI A Petrov
Jul 14, 2001·Molecular and Cellular Endocrinology·R Sakai, Y Eto
May 3, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A X LeJ A Helms
Jul 1, 1996·Bone·W V Giannobile
Jan 14, 2000·Kidney International. Supplement·T Freemont
Oct 20, 2000·Ceskoslovenská patologie·M ZámecníkP Gomolcák
Jun 5, 2010·Implant Dentistry·Dennis G SmilerConnie Matthews
Aug 1, 1997·European Journal of Oral Sciences·X SiF H White
Jan 26, 1999·Annals of the New York Academy of Sciences·C M FergusonJ A Helms
Aug 1, 1997·Molecular Pathology : MP·D M LawtonA J Freemont
Nov 1, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Sheng-Li HuangHong-Wei Yan
Oct 12, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y KobayashiH Sakai
Jul 17, 2001·Journal of Periodontology·L MarinucciP Locci
Apr 11, 1992·Lancet
Dec 6, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Francois N K KwongChristopher H Evans
Apr 5, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Laura Rose, Hasan Uludağ
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Vera LuginbuehlBruno Gander
Jul 16, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ashvin K DewanPaul D Sponseller
Jun 26, 2001·The Journal of Surgical Research·S M WarrenM T Longaker
Sep 21, 2000·The Tohoku Journal of Experimental Medicine·R S Yang, T F Huang
Jul 10, 2002·International Journal of Oral and Maxillofacial Surgery·K E YatesJ Glowacki
Oct 16, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Paul Kostenuik, Faisal M Mirza
Apr 20, 2004·The American Journal of Sports Medicine·Georg N DudaAndreas Weiler
Sep 19, 1997·Journal of Dental Research·W V GiannobileS E Lynch
May 1, 1997·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·A Probst, H U Spiegel
Jul 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M K DuncanS I Tomarev
Oct 1, 2009·Craniomaxillofacial Trauma & Reconstruction·Mohammed E Elsalanty, David G Genecov
Jan 26, 1999·Clinical Orthopaedics and Related Research·R N RosierD G Hicks
Aug 17, 2001·The Journal of Bone and Joint Surgery. American Volume·M L BouxseinJ M Wozney
Nov 14, 2002·The Journal of Bone and Joint Surgery. American Volume·Peter KloenDavid L Helfet

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