Demonstration of the functional role of conserved Glu-Arg residues in the Staphylococcus aureus ferrichrome transporter

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Enrique D VinésDavid E Heinrichs

Abstract

The features that govern the interaction of ligand binding proteins with membrane permeases of cognate ABC transporters are largely unknown. Using sequence alignments and structural modeling based on the structure of the Escherichia coli BtuCD vitamin B12 transporter, we identified six conserved basic residues in the permease, comprised of FhuB and FhuG proteins, in the ferrichrome transporter of Staphylococcus aureus. Using alanine-scanning mutagenesis we demonstrate that two of these residues, FhuB Arg-71 and FhuG Arg-61, play a more dominant role in transporter function than FhuB Arg-74 and Arg-311, and FhuG Arg-64 and Lys-306. Moreover, we show that at positions 71 and 61 in FhuB and FhuG, respectively, arginine cannot be substituted for lysine without loss of transporter function. Previously, our laboratory demonstrated the importance of conserved acidic residues in the ferrichrome binding protein, FhuD2. Taken together, these results support the hypothesis that Glu-Arg salt bridges are critical for the interaction of the ligand binding protein with the transmembrane domains FhuB and FhuG. This hypothesis was further studied by "charge swapping" experiments whereby we constructed a S. aureus strain expressing FhuD2 with co...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·R P Novick
Nov 10, 1995·The Journal of Biological Chemistry·J B Neilands
Jul 1, 1997·Perspectives in Biology and Medicine·E D Weinberg
Jun 2, 2000·Science·J E Posey, F C Gherardini
Oct 4, 2000·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·J R Chipperfield, C Ratledge
Sep 8, 2001·Science·C F Higgins, K J Linton
Aug 28, 2002·Biochemical Society Transactions·G Winkelmann
Dec 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth L BorthsDouglas C Rees
Oct 12, 2004·The Journal of Biological Chemistry·M Tom SebulskyDavid E Heinrichs
Dec 4, 2004·Journal of Bacteriology·Suzanne E DaleDavid E Heinrichs
Apr 9, 2005·The New England Journal of Medicine·Henry F Chambers
Feb 27, 2007·Nature·Kaspar HollensteinKaspar P Locher
Jul 1, 2008·Trends in Microbiology·Binh An Diep, Michael Otto
Oct 30, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kaspar P Locher
Oct 24, 2009·Journal of Inorganic Biochemistry·Federico C Beasley, David E Heinrichs
Apr 9, 2010·Natural Product Reports·Robert C Hider, Xiaole Kong
Jul 3, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Byron C ChuHans J Vogel

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Citations

Mar 18, 2015·Molecular Microbiology·Mélissa HannauerDavid E Heinrichs
Apr 12, 2015·FEMS Microbiology Reviews·Jessica R Sheldon, David E Heinrichs
Nov 5, 2019·Research in Microbiology·Philippe Delepelaire

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