Demonstration of the interaction between the CD23 molecule and the galactose residue of glycoproteins

Immunology Letters
S Kijimoto-OchiaiT Uede

Abstract

The CD23 molecule is a low-affinity receptor for IgE and has a marked homology in amino acid sequence with C-type animal lectins, including asialoglycoprotein receptor. We tested whether the CD23 antigen can indeed interact with the sugar chain of glycoproteins. Detergent extract of the membrane component from Epstein-Barr Virus (EBV)-transformed human B-cell line, L-KT9 cells, was incubated with asialofetuin (ASF)-coupled Sepharose, and bound proteins were effectively eluted by 0.3 M lactose or galactose which were among the competitive sugars tested. In this eluate, the CD23 molecule was detected by an immunoblotting technique. Because fetuin has both an N- and O-type sugar chain on the molecule, we tested which type of sugar chain can interact with CD23. The CD23 molecule interacted with asialocasein having a sugar chain with the Gal-GalNAc structure with asialobovine submaxillary mucin having the GalNAc structure, and also with ASF; however, it faintly interacted with ASF after removal of the O-type sugar chain by beta-elimination. These results showed that the CD23 molecule can, indeed, interact with the galactose residue, especially with the Gal-GalNAc rather than the Gal-GlcNAc structure of the terminal sugar chain of gl...Continue Reading

References

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Citations

Feb 16, 2000·Biochemical and Biophysical Research Communications·S Kijimoto-Ochiai, A Noguchi
Feb 1, 1997·European Journal of Biochemistry·H J Gabius
Apr 13, 2002·Annals of the New York Academy of Sciences·Grant R YeamanGillian A Lang
Aug 14, 2001·Biochemical and Biophysical Research Communications·K KotaniS Kijimoto-Ochiai
Dec 13, 2005·The FEBS Journal·Alex N Zelensky, Jill E Gready
Jul 28, 2009·Biochemical and Biophysical Research Communications·Toshiaki KodaJin-ichi Inokuchi
Nov 22, 1997·European Journal of Immunology·R Binsack, I Pecht
Apr 26, 2000·The Journal of Biological Chemistry·K AriizumiA Takashima
Jan 1, 1997·International Reviews of Immunology·M D MossalayiP Debré
Sep 7, 2019·The Journal of Biological Chemistry·Sabine A F JégouzoMaureen E Taylor

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