PMID: 6270015Mar 15, 1981Paper

Demonstration of the transferrin receptor in human breast cancer tissue. Potential marker for identifying dividing cells

International Journal of Cancer. Journal International Du Cancer
J E ShindelmanH H Sussman

Abstract

A transferrin receptor was demonstrated in tumor tissue from 10 patients with breast carcinoma and one patient with breast sarcoma. Binding studies were conducted by measuring the amont of 1251-transferrin binding to microsomal preparations of the tumor tissue. Elevated levels of specific transferrin binding were found in the tumors with a range of 11-35% of bound transferrin, whereas microsomes prepared from non-neoplastic breast tissue samples bound only 2.3% and 2.4% of the transferrin. Scatchard analysis of binding studies conducted with tissues from a breast cancer and from a breast sarcoma indicate that the receptor has a Ka = 9.0 x 10(8)M. The binding site is specific for transferrin, as studies show that non-radioactive transferrin displaced labelled transferrin, while human IgG and human albumin did not. The receptor-transferrin complex was precipitated from a detergent extract of the breast sarcoma with antiserum to human transferrin. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis of the immunoprecipitate gave a polypeptide of M 90,000 daltons, which is of similar molecular weight found for the putative transferrin receptor in all of a series of human cultured cell lines previously examined.

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T A HamiltonH H Sussman
Jan 1, 1979·Journal of Supramolecular Structure·J W Larrick, P Cresswell
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S E Hutchings, G H Sato
Jul 1, 1974·Experimental Cell Research·D Hemmaplardh, E H Morgan
Jan 1, 1980·Cellular Immunology·G M GalbraithW P Faulk

❮ Previous
Next ❯

Citations

Jan 1, 1985·Journal of Cancer Research and Clinical Oncology·B VandewalleJ Lefebvre
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·F WrbaJ H Holzner
Jan 1, 1986·Breast Cancer Research and Treatment·S E TonikH H Sussman
Jan 1, 1985·The Journal of Membrane Biology·W S May, P Cuatrecasas
Mar 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E Y LeeM J Bissell
Aug 1, 1992·Biological Trace Element Research·E D Weinberg
Nov 15, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·R TroccoliF Mannello
Jan 1, 1984·The International Journal of Biochemistry·A Hirose-KumagaiN Akamatsu
May 23, 1985·Journal of Immunological Methods·A H Lazarus, M G Baines
Apr 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·N FortunatiG Gaidano
Aug 1, 1996·Nuclear Medicine and Biology·L LangW C Eckelman
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Patrick P NgManuel L Penichet
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C A EnnsH H Sussman
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A EnnsH H Sussman
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I S Trowbridge, F Lopez
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P EkblomR Timpl
Feb 18, 2011·Thyroid : Official Journal of the American Thyroid Association·Gaetano MagroRoberto Perris
Jul 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sneha SundaramUday B Kompella
Dec 1, 1996·Cytopathology : Official Journal of the British Society for Clinical Cytology·P AthanassiadouK Kyrkou
Dec 8, 1989·European Journal of Biochemistry·D RoironC Figarella
Dec 1, 1984·The British Journal of Ophthalmology·R van der GaagT E Feltkamp
Jan 1, 1984·Journal of Clinical Pathology·R RossielloG G Giordano
Dec 14, 2012·American Journal of Physiology. Renal Physiology·Richard BouleyDennis Brown
Apr 1, 1990·Journal of Neurosurgery·W A HallR J Youle
May 1, 1992·Journal of Neurosurgery·W A HallO Fodstad
Jul 31, 2013·Biochimica Et Biophysica Acta·Ahmed A Alkhateeb, James R Connor
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T D OberleyP J Anderson
Jan 1, 1990·Drug Metabolism Reviews·E D Weinberg
Jan 1, 1986·Cancer Investigation·L M Neckers, J B Trepel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.