Demonstration of the translocation der(16)t(1;16)(q12;q11.2) in interphase nuclei of Ewing tumors

Genes, Chromosomes & Cancer
C M HattingerP F Ambros

Abstract

The der(16)t(1;16) has been detected cytogenetically in a number of malignancies including Ewing tumors (ETs). To enable fast and reliable analysis of der(16) chromosomes, we established an interphase cytogenetic approach. By using two DNA probes hybridizing to the heterochromatic portions on the long arms of chromosomes 1 and 16, this technique allows the detection of this chromosomal aberration in nonproliferating cells. Formation of the der(16) leads to partial excess of 1q material and partial loss of the long arm of chromosome 16. Double-target fluorescence in situ hybridization (FISH) experiments were performed on cytospin slides of 13 ETs, near-triploid tumor cells and normal cells to assess whether the FISH technique used permits the discrimination of nuclei harboring this aberration from nuclei without a der(16) chromosome. In five ETs, we found evidence for the presence of one or two der(16)t(1;16) chromosomes both by FISH and by conventional cytogenetics. Tumor cells displayed two signals for intact chromosomes 1, one or two additional fused signals for the der(16) chromosomes, and one signal for the intact chromosome 16. In one case without fused signals, the presence of a der(16) was demonstrated by hybridizing a p...Continue Reading

References

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Citations

Aug 19, 1999·Genes, Chromosomes & Cancer·C M HattingerP F Ambros
Sep 11, 2001·Genes, Chromosomes & Cancer·T OzakiB Dockhorn-Dworniczak
Jan 29, 2003·Cancer Genetics and Cytogenetics·Lian ZhaoArmand Glassman
Jul 24, 2003·The Journal of Molecular Diagnostics : JMD·Nathan C BirchJulia A Bridge
Dec 19, 2000·Histopathology·D GancbergD Larsimont
Oct 16, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·T OzakiC Poremba
Dec 8, 2009·Cancer Genetics and Cytogenetics·Monia LunghiGianluca Gaidano
Mar 14, 2007·Cancer Genetics and Cytogenetics·Kayla M KapelsJulia A Bridge
Feb 9, 1999·Medical and Pediatric Oncology·C M KullendorffN Mandahl
Jun 28, 2002·British Journal of Cancer·C M HattingerD R Betts

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