PMID: 8457321Jan 1, 1993Paper

Demonstration of toxin A and B by polymerase chain reaction and McCoy cell assay in clinical isolates of Clostridium difficile from Denmark

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
J D Knudsen, M Tvede

Abstract

A polymerase chain reaction (PCR) for demonstration of gene fragments of Clostridium difficile was established. One hundred and sixty-eight clinical isolates of C. difficile from three population groups were tested for production of cytotoxins by McCoy cell line assay (MCA) and for fragments of toxin A and B genes by PCR. The fragments for PCR amplification were at the 5' end of the toxin genes, which was found to be specific for C. difficile. Full agreement between the PCR and MCA results was found with respect to toxicity. Fifty-eight of the 168 strains were cytotoxin positive. Isolates from 41 normal healthy children did not differ regarding cytotoxicity compared to isolates from hospitalized children. In adult hospitalized patients a much higher frequency of toxin-producing isolates was found. In two strains from two children, not of the same family, only the toxin A gene fragment was demonstrated, indicating that some strains of C. difficile only harbour the gene for enterotoxin. When two isolates from different periods of time were tested from 36 of the healthy children, a variation in cytotoxicity was found: in seven children strains changed from non-toxic to toxic and in four vice versa. This may be explained by a fluct...Continue Reading

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Citations

Oct 19, 2010·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Frank M Aarestrup, Michael Tvede
Jan 7, 2011·Journal of Clinical Microbiology·Ruth Ann LunaJames Versalovic
Jun 1, 1996·Critical Reviews in Clinical Laboratory Sciences·D H Gröschel
Jan 24, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Sawsan TahaKarel Krovacek
Oct 16, 1999·The American Journal of Gastroenterology·C M Surawicz

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