Demonstration of viability and fertility and development of a molecular tool to identify YY supermales in a fish with both genotypic and environmental sex determination

Ecology and Evolution
R S HattoriYoji Yamamoto

Abstract

The pejerrey possesses a genotypic sex determination system driven by the amhy gene and yet shows marked temperature-dependent sex determination. Sex-reversed XY females have been found in a naturally breeding population established in Lake Kasumigaura, Japan. These females could mate with normal XY males and generate YY "supermale" individuals that, if viable and fertile, would sire only genotypic male offspring. This study was conducted to verify the viability, gender, and fertility of YY pejerrey and to develop a molecular method for their identification. Production of YY fish was attempted by crossing a thermally sex-reversed XY female and an XY male, and rearing the progeny until sexual maturation. To identify the presumable YY individuals, we first conducted a PCR analysis using amhy-specific primers to screen only amhy-positive (XY and YY) fish. This screening showed that 60.6% of the progeny was amhy-positive, which suggested the presence of YY fish. We then conducted a second screening by qPCR in order to identify the individuals with two amhy copies in their genome. This screening revealed 13 individuals, all males, with values twice higher than the other 30 amhy-positive fishes, suggesting they have a YY complement. ...Continue Reading

References

Jan 1, 1975·The Journal of Heredity·T O Yamamoto
Mar 1, 1988·The Journal of Heredity·B ChevassusB Jalabert
Oct 14, 2008·Conservation Biology : the Journal of the Society for Conservation Biology·Samuel Cotton, Claus Wedekind
Feb 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ricardo S HattoriCarlos A Strüssmann
Apr 23, 2013·General and Comparative Endocrinology·Ricardo Shohei HattoriGustavo Manuel Somoza
Jun 19, 2013·General and Comparative Endocrinology·Juan I FernandinoGustavo M Somoza
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·A Ross BrownCharles R Tyler
Jun 15, 2017·G3 : Genes - Genomes - Genetics·Dilip Kumar BejYoji Yamamoto

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Citations

Mar 27, 2019·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·Munti SaridaCarlos A Strüssmann

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Methods Mentioned

BETA
biopsy
PCR
genotyping

Software Mentioned

R

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