Oct 15, 1996

Demonstration that the removal of sialic acid from the surface of chicken spermatozoa impedes their transvaginal migration

Theriogenology
M G Steele, G J Wishart

Abstract

Staining of ejaculated chicken spermatozoa with lectin from FITC-conjugated Limulus polyphemus indicated a uniform distribution of terminal sialic acid residues on surface-associated glycoproteins throughout all regions of the spermatozoa. Treatment of spermatozoa with neuraminidase resulted in complete disappearance of this lectin affinity without any visible change in the viability of spermatozoa, as indicated by assessment in vitro of their motility, ATP content and ability to exclude eosin. Neuraminidase-treated spermatozoa were also severely limited in their ability to populate the uterovaginal sperm storage tubules after intravaginal insemination, although they were able to perform this function as well as untreated control spermatozoa after insemination directly into the uterovaginal region. These results demonstrate the presence of a mechanism that recognises sperm surface characteristics which inhibit transport of spermatozoa through the vagina of the chicken hen, but not the entry of spermatozoa into the sperm storage tubules. We hypothesize that cleavage of sperm surface sialic acid residues may increase antigenicity of spermatozoa in the vagina, resulting in their destruction by an immunologically-based sperm-select...Continue Reading

  • References16
  • Citations10

Citations

Mentioned in this Paper

Cell Motility
Glycoproteins
Sperm Cell
Spermatozoa Cell Count
Cytokinesis of the Fertilized Ovum
Motility
Sialic Acid Assay
Eosine Yellowish
Neuraminidase
Sperm Retention

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Head And Neck Squamous Cell Carcinoma

Squamous cell carcinomas account for >90% of all tumors in the head and neck region. Head and neck squamous cell carcinoma incidence has increased dramatically recently with little improvement in patient outcomes. Here is the latest research on this aggressive malignancy.

Signaling in Adult Neurogenesis

Neural stem cells play a critical role in the production of neuronal cells in neurogenesis is of great importance. Of interest is the role signalling mechanisms in adult neurogenesis. Discover the latest research on signalling in adult neurogenesis.

Psychiatric Chronotherapy

Psychiatric Chronotherapy considers the circadian rhythm as a major factor for optimizing therapeutic efficacy of psychiatric interventions. Discover the latest research on Psychiatric Chronotherapy here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.