PMID: 6988667Jan 1, 1980Paper

Denaturation mapping of the ribosomal DNA of Saccharomyces cerevisiae

Molecular & General Genetics : MGG
J H Cramer, R H Rownd

Abstract

The thermal melting profile of purified Saccharomyces cerevisiae ribosomal DNA (rDNA) is biphasic indicating considerable intramolecular heterogeneity in base composition. The first phase of the transition, about 20% of the total hyperchromic shift, has a Tm of 80.6 degrees C and the second phase has a Tm of 87.3 degrees C, corresponding to GC contents of 28 and 44%, respectively. The Tm of the nonribosomal nuclear DNA, called alpha DNA, is 85.7 degrees C. This heterogeneity in GC distribution in the rDNA is also reflected in its denaturation map. A denaturation map of the 5.6 X 10(6) dalton rDNA SmaI restruction fragment, which represents monomer units of the rDNA, shows that specific regions of the repeating unit denature more readily than the remainder and apparently have a significantly higher AT content. By aligning the rDNA denaturation map with the restriction endonuclease map, we have been able to determine that the AT-rich segments are localized in the transcribed and nontranscribed spacer regions of the rDNA repeating unit. Buoyant density determinations of individual rDNA restriction fragments corroborate the locations of AT-rich regions. A denaturation map of the tandem repeating units in higher molecular weight rDN...Continue Reading

References

Jun 16, 1977·Nature·A M MaxamW Gilbert
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Citations

Oct 11, 1982·Nucleic Acids Research·O S Gabrielsen, T B Oyen
Mar 26, 1984·Nucleic Acids Research·K G SkryabinA A Hadjiolov
Jun 29, 2017·Archives of Endocrinology and Metabolism·Willian Dos SantosLupe Furtado-Alle

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